中文摘要：

将CaMV35S启动子驱动的内质网小分子热激蛋白基因导入番茄，比较转基因、未转基因和转空载体番茄的抗冷能力。冷胁迫下，同对照相比，转基因番茄的冷害症状轻，叶绿素含量的损失少，体内积累的丙二醛（MDA）含量少，电解质外渗程度低，具有较高的净光合速率和最大光化学效率，并易于恢复由低温所引起的光抑制，表明转基因番茄具有较强的冷胁迫耐性，说明内质网小分子热激蛋白在植物抗冷过程中发挥重要作用。

英文摘要：

The full-length endoplasmic reticulum small heat shock protein （ER-sHSP） cDNA using CaMV 35S promoter was transformed into the genome of tomato plants. The chilling tolerance of transgenic tomato plants, non-transgenic tomato plants and pROK Ⅱ-transformed tomato plants were studied. Under chilling stress treatment, compared with non-transgenic and pROK Ⅱ-transformed tomato plants, transgenic tomato plants exhibited slighter cold-injured symptoms, suffered less destruction of chlorophyll and electrolyte leakage. The content of MDA of transgenic tomato plants was lower than that of non-transgenic and pROK Ⅱ -transformed tomato plants, and transgenic tomato plants could keep up higher value of net photosynthetic rate, Fv/ Fm and could recover quickly from chilling-induced photosynthetic inhibition. The results showed that ER- sHSP played a key role in enhancing the chilling-resistance ability of plants.