柑橘黄龙病(Huanglongbing)由韧皮部杆菌属细菌(Candidatus Liberobactersp.)侵染引起,是柑橘生产上的毁灭性病害,当前正在世界柑橘产区迅速蔓延,已成为制约柑橘产业健康发展的关键因素之一。介绍了柑橘黄龙病的症状以及病原的分离培养研究进展,阐述了病原的分子检测技术,包括PCR、双重PCR、实时荧光定量PCR和LAMP快速检测技术,并对病害的防控进行展望。
Citrus greening disease (Huanglongbing) caused by bacterial (Candidatus Liberobacter sp.), is a devastating disease of citrus production, is currently spreading rapidly in citrus producing areas of the world and has become a key constraint factor of the citrus industry. This article describes the symptoms of citrus Huanglongbing disease and pathogen isolation and culture, a detailed molecular pathogen detection technology, including PCR, double-PCR, real-time PCR and LAMP rapid detection technology, and made the prospect of disease prevention and control.