在亚硒酸存在的条件下,采用干燥加热的方法对魔芋葡甘露寡糖(KOS)进行硒酸化;并用红外光谱、紫外光谱、X射线衍射等技术对硒化魔芋葡甘露寡糖(Se-KOS)进行表征,同时测定Se-KOS的抗氧化性。红外光谱实验结果表明:Se-KOS在890cm-1附近出现Se=O的吸收峰,证明硒已被成功接到KOS中。与KOS相比,Se-KOS的ABTS+.清除能力、DPPH自由基清除能力、还原能力和Fe2+螯合能力等体外抗氧化能力均有显著增强,证明硒酸化对提高KOS的抗氧化性来说是一种很有效的方法。
In the present study,konjac-glucomannan oligosaccharides(KOS) were selenized by dry-heating in the presence of selenite,and selenized KOS(Se-KOS) were characterized by ultraviolet,infrared(IR) and X-ray diffraction spectroscopy.Furthermore,the in vitro antioxidant capacity of Se-KOS was evaluated.The IR spectrum of Se-KOS showed a strong band at 890 cm-1 attributed to Se=O,suggesting that selenium has been successfully conjugated with KOS.The DPPH radical scavenging activity,ABTS+· scavenging activity,Fe2+ chelating activity,and reducing power of Se-KOS were markedly enhanced due to selenization.Our results suggest that selenization is an efficient method for enhancing the antioxidant activity of KOS and thus may provide a new method for manufacturing organic Se and antioxidant.