中文摘要：

目的：探讨miR-34a在幼鼠海马神经元细胞增殖凋亡中的作用.方法：分离幼鼠海马神经元细胞,转染miR-34a抑制剂（miR-34a inhibitor）、抑制剂对照（inhibitor control）、miR-34a模拟物（miR-34a mimics）、模拟物对照（mimics control）,RT-PCR检测细胞中miR-34a表达水平.MTT检测转染后细胞增殖情况.流式细胞仪检测细胞凋亡情况.Western blot检测细胞中Cleaved-caspase-3、Bcl-2、Bax的表达水平.结果：转染miR-34a inhibitor可以抑制miR-34a的表达,miR-34amimics可以促进miR-34a的表达.miR-34a mimics对细胞增殖抑制率明显高于mimics control组（P＜0.05）,miR-34a inhibitor组抑制率明显低于inhibitor control组（P＜0.05）.miR-34a inhibitor组神经元细胞凋亡率明显低于inhibitor control组（P＜0.05）,miR-34a mimics组神经元细胞凋亡率明显高于mimics control组（P＜0.01）,inhibitor control组和mimics control组神经元细胞凋亡率差异不显著（P＞0.05）.miR-34a inhibitor组Cleaved-caspase-3、Bax蛋白表达量低于inhibitor control组,差异显著（P＜0.05）;miR-34a inhibitor组Bcl-2蛋白表达量高于inhibitor control组,差异显著（P＜0.05）;miR-34a mimics组Cleaved-caspase-3、Bax蛋白表达量高于mimics control,差异显著（P＜0.05）;miR-34a mimics组Bcl-2蛋白表达量低于mimics control,差异显著（P＜0.05）.结论：miR-34a抑制海马神经元细胞增殖,促进细胞凋亡,其作用机制可能与调控Cleaved-caspase-3、Bcl-2、Bax表达有关.

英文摘要：

Objective：To investigate the role of miR-34a on proliferation and apoptosis of hippocampal neurons cell.Metlhods：Isolated rats hippocampal neurons were transfected with miR-34a inhibitor,inhibitor control,miR-34a mimics,mimics control,respectively.RT-PCR was used to detect the expression level of miR-34a,and MTT was used to detect cell proliferation.Apoptosis was detected by flow cytometry.Western blot analysis was determined the expression levels of Cleaved-caspase-3,Bcl-2,Bax.Results：Transfection with miR-34a inhibitor could inhibit the expression of miR-34a,miR-34a mimics could promote the expression of miR-34a.The cell proliferation inhibition rate in miR-34a mimics group was significantly higher than that of mimics control group （P ＜0.05）,and the inhibition rate in miR-34a inhibitor group was significantly lower than that of inhibitor control group （P ＜0.05）.The neuronal apoptosis in miR-34a inhibitor group was significantly lower than that of inhibitor control group （P ＜0.05）,and the neuronal apoptosis was significantly higher in miR-34a mimics group than that of mimics control group （P ＜0.01）.The neuronal apoptosis rate was not significantly different between inhibitory control group and mimics control group （P＞ 0.05）.The cleaved-caspase-3,Bax protein expression level lower in miR-34a inhibitor group than that of inhibitor control group,and the difference was significant （P ＜0.05）;and Bcl-2 protein expression was higher in miR-34a inhibitor group than that of inhibitor control group,and the difference was significant （P ＜0.05）.The cleaved-caspase-3,Bax protein expression was higher in miR-34a mimics group than that of mimics control with statistical significance （P ＜0.05）;and Bcl-2 protein expression of miR-34a mimics group was lower than that of mimics control （P ＜0.05）.Conclusions：miR-34a can inhibit proliferation of hippocampal neurons and promote neuronal apoptosis,and its mechanism may be related to Cleaved-caspase-3,Bcl-2,Bax.