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透骨消痛颗粒含药血清对兔软骨细胞增殖影响的研究

ISSN号：1009-2501
期刊名称：《中国临床药理学与治疗学》
时间：0
分类：R965.2[医药卫生—药理学;医药卫生—药学]
作者机构：[1]福建中医学院中西医结合研究院,福建福州350108, [2]福建省高校中西医结合基础重点实验室,福建福州350108, [3]福建省中西医结合老年性疾病重点实验室,福建福州350108
相关基金：国家自然科学基金项目（30672701）;福建省自然科学基金项目（2009J01172/200800098）;福建省中西医结合老年性疾病重点实验室开放课题基金资助（2008J1004.27）;陈可冀中西医结合发展基金（CKJ2008005/2008025/2007022/2009006）

作者：叶蕻芝[1,2], 李西海[3], 梁文娜[3], 郑春松[2], 陈文列[2], 刘献祥[1]

关键词：透骨消痛颗粒, 含药血清, 细胞增殖, 细胞周期, Tougu Xiaotong Granula（TXG）, serum containing drug, cell multiplication, cell cycle

中文摘要：

目的：探讨透骨消痛颗粒含药血清对兔软骨细胞增殖的影响。方法：取4周龄新西兰兔膝关节关节软骨Ⅱ型胶原酶消化,建立软骨细胞体外培养体系,体外培养第3代软骨细胞随机分为5组,分别加入培养液、空白血清和中药血清低、中、高剂量继续培养24、48、72 h后,采用流式细胞仪检测软骨细胞周期的变化。结果：流式细胞仪细胞周期检测显示中药血清中、高剂量能够刺激软骨细胞由G0/G1期进入S期和G2/M期,并呈现时间的依赖性,干预48 h后G0/G1期细胞比例降低,S期与G2/M期细胞之和增加,与低量组、空白组相比差异有统计学意义（P〈0.05）。结论：透骨消痛颗粒含药血清能有效促进软骨细胞的增殖。

英文摘要：

AIM： To investigate the effects of Tougu Xiaotong Granula（TXG）-containing serum on proliferation of rabbit chondrocytes in vitro. METHODS： The chondrocyte was obtained from the arthrodial cartilage of 4-week-old New Zealand rabbit by collagenase n digestion, so as to establish the system of chondroeyte culture in vitro. The 3rd passage of chondrocyte in vitro was randomly divided into five groups which were respectively cultured with medium, medium with serum and the high, medium, low doses of TXG-containing serum. After cultured for 24,48 and 72 hours, the cell cycle changes were detected by the flow cytometry. RESULTS： The high, medium doses of TXG- containing serum could stimulate the chondrocyte through G0/G1 phases into S and G2/M phases in a time-dependent manner by flow cytometry. Compared with the control and low-dose groups, the proportions of cells in G0/G1 phase in the high- and medium-dose groups were significantly lower, meanwhile those in S and G2/M phases were significantly higher after 48 hours（ P 〈 0.05）. CONCLUSION： TXG-containing serum could effectively promote the cell proliferation of chondrocytes.

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