中文摘要：

目的：研究黄连素（BBR）对 A549细胞增殖、迁移和侵袭的影响，初步探讨 BBR 抑制 A549细胞转移的机制。方法：用不同浓度的 BBR 作用于 A549细胞，MTT 法测定 A549细胞增殖水平，流式细胞仪检测 A549细胞的凋亡，Transwell 实验测定 A549细胞的迁移和侵袭能力，Western blot 测定 A549细胞中与 EMT 相关的蛋白 E - cadherin、Vimentin、Slug、Snail 的表达。结果：BBR 以浓度及时间依赖的方式抑制 A549细胞的增殖；流式细胞仪检测发现 BBR 作用于 A549细胞后凋亡率呈剂量依赖性增加；Western blot 显示 BBR 作用 A549细胞后，E - cadherin 表达升高，Vimentin、Slug、Snail 表达降低，Transwell 实验显示 BBR 作用于 A549细胞后，能够抑制其迁移和侵袭。结论：BBR 能抑制 A549细胞增殖，诱导其凋亡，通过促进 E - cadherin 表达和抑制Vimentin、Slug、Snail 的表达来抑制 EMT 的发生，从而抑制了 A549细胞的迁移和侵袭。

英文摘要：

Objective：To study the effect of Berberine on Proliferation,migration and invasion of A549 cells. Methods：The Proliferation and aPoPtosis of A549 cells were determined by MTT and FACS resPectively. The rate of A549 cell migration was observed by Transwell assay. The exPression of E - cadherin,Vimentin,Slug,Snail in A549 cells effected by Berberine were assessed by Western blot. Results：The Proliferation of A549 cell could be significant-ly inhibited within limited duration and dosage（P ﹤ 0. 05）. Also exPression of Vimentin,Snail and Slug of gene were decreased but E - cadherin increased after Berberine treatment（P ﹤ 0. 05）. Conclusion：Berberine can inhibit the mi-gration abilities of A549 cells that might be related to down - regulating the exPression of Vimentin,Snail and uP -regulating the exPression of E - cadherin.