中文摘要：

目的探讨胰岛素样生长因子1受体（IGF-1R）基因G3174A位点多态性与广西巴马长寿现象的相关性。方法应用聚合酶链反应一限制性片段长度多态性（PCR—RFLP）技术对广西巴马长寿地区187名长寿老人（年龄90～110岁，长寿组）、该地区224名与长寿老人无血缘关系的健康成年人（年龄24～79岁，内对照组）以及一般地区187名健康成年人（年龄21～79岁，外对照组）的IGF-1R基因进行基因分型，并用直接测序法进行验证。比较长寿组与各对照组的基因型和等位基因频率的差异。结果长寿组GG、GA和AA三种基因型频率分别为41．2％，45．4％和13．4％；G和A等位基因频率分别为63．9％和36．1％。长寿组GA、AA基因型频率高于内对照组，差异有统计学意义（P〈0．05）；长寿组与外对照组的GA、AA基因型频率比较，差异无统计学意义。长寿组A等位基因频率及携带A等位基因的个体（IGF-1RA＋）所占构成比均高于内对照组、外对照组，差异有统计学意义（P〈0．05）。长寿组男性的GA、AA基因型频率高于内对照组，A等位基因频率均高于内、外对照组，差异均有统计学意义（P〈0．05）。长寿组女性的GG、GA、AA基因型频率和G、A等位基因频率与内、外对照组比较，差异均无统计学意义。与内、外对照组比较，长寿组GA、AA和GA＋AA基因型的OR值显著升高（P〈0．05），长寿组A等位基因的OR值亦显著升高（P〈0．05）。结论IGF-1R基因G3174A位点多态性与广西巴马男性长寿现象存在一定的关系，A等位基因可能是巴马长寿的有利因素。

英文摘要：

Objective To investigate the relationship between the insulin-like growth factor 1 receptor （IGF-1R） gene G3174A polymorphism and longevity in Bama, Guangxi province. Methods The IGF-1R genotypes were analyzed with PCR- RFLP and direct sequencing technique in 187 healthy long-fived elderly （aged 90-110, longevity group）, 224 local residents （aged 24-79, intra-controt group） from Bama county and 187 persons （aged 21-79, extra-control group） from general district. The differences in genotype and allele frequency between the longevity group and the control groups were compared. Results The frequencies of GG, GA, AA genotype, G and A allele in longevity group were 41.2% ,45.4%, 13.4%, 63.9% and 36.1%, respectively. The frequencies of GA and AA genotype in longevity group were significantly higher than those of the intra-control group （P〈0.05）, but no significant difference was found between longevity group and extra-control group （P〉0.05）. The percentages of GA＋AA genotype and A allele （IGF-1R A＋）in longevity group were significantly higher than those of the control groups （P〈0.05）.The GA and AA genotype were found more frequent in male long-lived elderly than those in intra-control group （P〈0.05）. The frequencies of A allele in male long-lived elderly were significantly higher than those of the control （P〈 0.05）. There were no significant differences in the frequencies of GG, GA, AA genotype, G and A allele between the female long-lived elderly and the control （P〉0.05）. It also showed the longevity positively correlated with GA,AA,GA＋AA genotype and A allele. Conclusion The IGF-1R gene G3174A polymorphism may be associated with male longevity in Bama, and the A allele of IGF-1R may be a protective factor.