朱鹮为世界濒危物种、用免疫组织化学方法对朱鹮脑内听觉一发声中枢和内分泌核团中的P物质免疫阳性神经细胞、终末和纤维进行了定位:1)在听觉通路中的延髓耳蜗核、脑桥腹部外侧丘系核、中脑背外侧核壳、中脑丘间核壳、丘脑卵圆核壳等有P物质阳性纤维和终末;在脑桥橄榄核有P物质阳性神经细胞;2)在发声通路中的气管呜管亚核、中脑丘间复合体背内侧核、丘脑前背外侧核内部、古纹状体腹部中间区有P物质阳性神经细胞和终末;3)在视前区前核、下丘脑外侧核,下丘脑腹内侧核等内分泌核团有P物质阳性神经细胞。结果提示:P物质在听觉通路和听觉旁通路、发声通路和生殖内分泌相关的核团中表达丰富,鸟脑中的P物质可能参与听觉和呜叫行为以及交配和生殖等生理功能的调制。
The distribution of substance P in the brain of Nipponia nippon was investigated by use of immunohisto- chemical methods, and the gray density of numerical value was measured with the image processing system. 1 )The sub- stance P labeled terminal and cells were distributed in the auditory center such as the rico, superior olivary nucleus (SO), nucleus ventralis lemnisci lateralis (VLV), the shell regions of mesencephalicus lateralis, pars dorsalis (MLd shell), the nucleus ovoidalis shell (Or shell) et al; 2)In the vocal control nuclei such as the nucleus hypoglossi, pars tracheosyringealis (nXIIts), nucleus dorsalis medialis (DM) and the nucleus intercollicularis (ICo), dorsolateral nucle- us of the anterior thalamus (DLM), AiV of Nipponia nippon. 3)In the endocrine nuclei such as nucleus ventromedialis hypothalamic (VMN), nucleus lateralis hypothalamic (LHy), nucleus preopticus anteror (POA) et al; The results indicate that substance P may play an important physiological role in the auditory-vocal-endocrine hypothalamic axis.