中文摘要：

为探讨肠炎沙门菌感染对鸡盲肠组织甲基化的影响，本研究选用2日龄肠炎沙门菌阴性寿光鸡和SPF白来航为试验材料，用肠炎沙门菌进行接种，接种后第1、3、14和21天采集盲肠组织样品提取DNA，进行甲基化水平检测。结果显示，肠炎沙门菌感染后第3天和第14天，寿光鸡基因组甲基化水平显著降低，感染后不同时间点，对照组和试验组甲基化水平均降低，且第21天对照组甲基化水平显著低于第1、3和14天；感染后各时间点，SPF鸡盲肠组织甲基化水平均低于对照组，但没有达到显著性水平，感染后不同时间点，对照组和试验组的甲基化水平均有所升高。本研究初步揭示了肠炎沙门菌感染抑制盲肠组织全基因组甲基化，这种抑制作用受遗传背景和感染后时间的影响，为今后基因组甲基化在沙门菌感染中的调控机制提供了理论依据。

英文摘要：

In order to investigate the effects of Salmonella enteritidis infection on chicken cecal genomic methylation,two-day-old with Shouguang chicken and SPF white Leghorn with were selected and inoculated with Salmonella enteritidis. The cecal genomic DNA methylation level was detected on day 1,3,14 and 21 after Salmonella enteritidis inoculation. Results showed that genomic DNA methylation level was decreased significantly on day 3 and 14 after inoculation in Shouguang chicken, at different time points after infection in the control and treated groups, which of control group on day 21 after infection was significantly lower than that on day 1,3 and 14 after infection. Cecal genomic DNA methylation level in the treated group was declined from day 1 to day 21 after infection in SPF chicken compared with control groups without statistical significance. At different time points after infection, methylation capacity in SPF chicken increased slightly in the control groups and the treated groups, respectively. This study initially revealed that Salmonella enteritidis infection inhibits the chicken cecal genomic DNA methylation level,which was influenced by genetic background and time points after Salmonella enteritidis infection. The results herein provided a theoretical foundation for the mechanism of genomic DNA methylation in the regulation of Salmonella enteritidis infection for further study.