中文摘要：

灰飞虱的群体带毒率及体内条纹病毒（rice stripe virus,RSV）的准确检测是预测水稻条纹叶枯病在CO2浓度升高背景下能否大发生的重要参考依据之一。本研究应用quali-fiedRT-PCR（qRT-PCR）测定不同CO2浓度条件下灰飞虱体内的RSV含量。结果表明：在试验的3个CO2浓度梯度（370、470和570μl.L-1）下,470μl.L-1的CO2浓度最适合RSV在灰飞虱体内的增殖,此方法与现已普遍应用的酶联免疫检测法（enzyme-linkedi mmu-nosorbnent assay,ELISA）和斑点免疫结合检测法（dotimmunobinding assay,DIBA）相比,具有快速、灵敏和特异性强的优点,且不需制备抗血清,适于普及应用。

英文摘要：

Accurate detection of populations virus-carrying rate and individuals rice stripe virus （RSV） content of small brown planthopper （Laodelphax striatellu） is one of the important references to predict whether the rice stripe disease could outbreak under elevated CO2 concentration. In this study,qualified RT-PCR assay （qRT-PCR） was employed to detect the RSV content in L. striatellu under different CO2 concentrations. Among the test three CO2 concentrations （370,470,and 570 μl·L-1）,470 μl·L-1 of CO2 was most favorable to the multiplication of RSV in L. striatellu. Compared with the commonly used ELISA （enzyme-linked immunosorbnent assay） and DIBA （dot immunobinding assay）,qRT-PCR was more rapid,sensitive,and specific,and didn＇t need antiserum preparation,being easily to be popularized.