中文摘要：

目的：观察不同培养液及不同培养时期等因素对体外培养人脑胶质瘤细胞CD133阳性（CD133^＋）细胞比率的影响。方法：用干细胞培养液（含EGF、FGF-2、B27）培养6例原代胶质瘤细胞和3例对照细胞株，用流式细胞仪检测培养第0、3、7、28、60、90和120天时不同细胞的CD133^＋细胞比率；免疫组化观察胶质瘤干细胞的多向分化能力；用血清、无血清和干细胞3种培养液分别培养2例长期培养的胶质瘤SHG62、SHG66细胞系以及对照的U87和U251细胞株，观察胶质瘤中CD133^＋细胞在不同培养液中的相互转换。结果：胶质瘤CD133^＋细胞比率随培养时间推移明显下降（P〈0．05），1周左右均降至〈1％的低水平，而细胞株则比较稳定；干细胞培养液培养的细胞中CD133^＋细胞比率明显高于血清和无血清培养液培养（P〈0．01）；此外，胶质瘤细胞中的干细胞具有多向分化的能力，并且可以在干细胞和血清培养液中互相转换。结论：原代胶质瘤体外培养过程中CD133^＋细胞比率逐渐下降，而干细胞培养液能明显提高CD133^＋细胞比率，并且CD133^＋细胞可以在不同培养液中相互转换。说明生长环境对干细胞比率影响较大，提示可能存在尚未明了的影响干细胞生长分化的相关因子。

英文摘要：

Aim ： To find the dynamic changes of CD133^＋（CD133 positively expressed） cells in primary culture and also in different mediums. Methods： Six primary malignant glioma cells and 3 glioma cell strains were cultured in stem cell medium （supplemented with EGF, FGF-2 and B27）, then their ratios of CD 133^＋ cells were checked with flowcytometry on day 0,3,7,28,60,90,120 respectively. Multiple differentiations of stem cells were proved by immunocytochemistry. Then changes of the ratios of CD133^＋ cells of SHG62, SHG66 （long-term cultured in our laboratory） and U87, U251 cell strains cultured in medium with or without serum and stem cell medium were observed. Results： The ratios of CD133^＋ cells in primary gliomas decreased evidently with time（P〈0.05）, while cell strains remained stationary levels. The ratio of CD 133^＋ cells was higher in stem cell medium than in serum-supplemented medium and serum-free medium. Conclusion： The ratios of CD133^＋ cells dropped with time in primary gliomas, and stem cell mediums were favored for CD133^＋ cells. And the cells could be switched successfully between serum-free medium and stem cell medium, which hints that there might be some undiscovered factors for stem cells thriving.