中文摘要：

瞄准：在食道的有鳞的房间癌(ESCC ) 调查 miRNA 的表示侧面。方法：在 ESCC 纸巾的 miRNA 的表示侧面被 miRNA microarray 分析。在 86 个 ESCC 病人的 miR-143 和 miR-145 的表示层次被即时聚合酶链反应(PCR ) 用 TaqMan 试金决定。活动性效果被愈合创伤的使用的食道的癌房间 transfected 与 miRNA 表示 plasmids 估计。结果：一套 miRNAs 被发现是在 ESCC 纸巾，和 miR-143 的表示层次的 deregulated， -145 显著地在大多数检验的 ESCC 纸巾被减少。miR-143 和 miR-145 表示与肿瘤侵略深度相关。然而，有 miR-143 和 miR-145 表示 plasmids 的人的食道的癌房间的 transfection 导致了房间活动性的更大的抑制 miR-145 的以前报导的目标的蛋白质水平， FSCN1，没显示出任何重要 downregulation。结论：这些调查结果建议 miRNAs 的解除管制在 ESCC 的前进起一个重要作用。miR-143 和 miR-145 可能充当对 ESCC 普通的 anti-oncomirs。

英文摘要：

AIM：To investigate the expression profile of miRNA in esophageal squamous cell carcinoma（ESCC）.METHODS：The expression profile of miRNA in ESCC tissues was analyzed by miRNA microarray.The expression levels of miR-143 and miR-145 in 86 ESCC patients were determined by real-time polymerase chain reaction（PCR） using TaqMan assay.The mobility effect was estimated by wound-healing using esophageal carcinoma cells transfected with miRNA expression plasmids.RESULTS：A set of miRNAs was found to be deregulated in the ESCC tissues,and the expression levels of miR-143 and-145 were significantly decreased in most of the ESCC tissues examined.Both miR-143 and miR-145 expression correlated with tumor invasion depth.The transfection of human esophageal carcinoma cells with miR-143 and miR-145 expression plasmids resulted in a greater inhibition of cell mobility,however,the protein level of the previously reported target of miR-145,FSCN1,did not show any significant downregulation.CONCLUSION：These findings suggest that the deregulation of miRNAs plays an important role in the progression of ESCC.Both miR-143 and miR-145 might act as anti-oncomirs common to ESCC.