中文摘要：

目的:观察角叉菜炎症时,5-羟色胺2A(5-HT_2A)受体mRNA在中缝大核（NRM）表达的水平及其与5-羟色胺（5-HT）免疫阳性神经元的共存。方法:实验采用原位杂交和荧光原位杂交结合荧光免疫组织化学双标技术。结果:正常大鼠NRM内有中等水平的5-HT_2A受体mRNA表达;角叉菜致炎后,5-HT_2A受体mRNA在双侧NRM内的表达明显增加,3-8h达峰值,24h后显著降低。在正常大鼠的NRM内,仅有少量5-HT_2A受体mRNA和5-HT免疫阳性双标神经元,双标神经元的数量在角叉菜致炎后无明显变化。结论:NRM内5-HT_2A受体主要分布在非5-HT神经元,且随外周炎症的发展,5-HT_2A受体的合成增加。

英文摘要：

AIM: Examine the expression of 5-HT2A receptor mRNA and co-localization of 5-HT2A receptor mRNA with 5-HT immunoreactivity in nucleus raphe magnus (NRM) neurons following carrageenan inflammation. METHODS: In situ hybridization (ISH) and combining fluorescent ISH with immunofluorescent histochemical double staining technique were used in the present study. RESULTS: 5-HT2A receptor mRNA was expressed in moderate level in NRM neurons. Following carrageenan inflammation, the expression of 5-HT2A receptor mRNA in NRM was greatly increased. The peak occurred at 3 - 8 h and then there was a clear decrease at 24 h after injection of carrageenan. Only a few 5-HT2A receptor mRNA and 5-HT immunoreactive double labeled cells were observed in NRM, and the number of the double labeled cells had no obvious change following carrageenan inflammation. CONCLUSION: The synthesis of 5-HT2A receptor was increased in non-serotonin rather than serotonin neurons of NRM during peripheral inflammation.