中文摘要：

目的考察补骨脂丙酮提取物的抗骨质疏松作用及其机制。方法采用大鼠去卵巢骨质疏松模型，通过影像学、形态学、分子生物学方法考察补骨脂丙酮提取物的抗骨质疏松作用及其机制。结果模型组的骨密度明显下降（P〈0．05）；而H0高、中剂量组则显著提升（P〈0．05）。HE染色显示：模型组股骨骨小梁遭到严重破坏，而仙灵骨葆组、H0高、中剂量组骨小梁网状结构的完整性好。荧光定量PCR显示，模型组股骨β-ERmRNA水平与对照组没有显著差异。结论补骨脂丙酮提取物H0组分高、中剂量组初步显示抗骨质疏松特性，其机制与骨组织里D型雌激素受体无关。

英文摘要：

Objective To study preventive effects and mechanism ofPsoralen acefone extract on osteoporosis. Methods Ovariectomized rats were used as the model of osteoporosis,and preventive effects and mechanism of Psoralen acetone extract on osteoporosis were investigated by the methods of radiology, morphology, molecular biology.Result The BMD of model group decreased significantly（P〈0.05）, while H0 groups with high dose and middle dose were significantly increased （P〈0.05）. HE staining showed that： femoral trabeeular bone of model group was severely damaged, but that of Xianlinggubao group, H0 groups with high dose and middle dose had kept a well shape of mesh. Fluorescent quantitation PCR showed that right femur of rats of β-type estrogen receptor mRNA levels were not significantly different comparing with the control group. Conclusion The Psoralen acetone extract H0 at high dose and middle dose showed effects against osteoporosis of rats. The mechanism of anti-osteoporosis of H0 component had nothing to do with the β-type estrogen receptor in osteoblasts.