中文摘要：

在在海南省在 Boluo，在广东省的 Huilai 县和 Lingshui 县种的野米饭 Oryza rufipogon Griff 开拓殖民地于的 endophytic diazotrophs 的差异和 nitrogenase 活动被学习。37 孤立从 Oryza 获得了 rufipogon 当由 ARA (乙炔减小试金) 的修理氮的细菌测试并且推进的通常认为的 endophytic 由 nifH 基因碎片的 PCR 扩大证实了，被识别。所有获得的紧张举办 ARA 活动和一样的大小的 nifH 基因碎片。在 80% 的类似水平上面，获得孤立作为我采指纹到由聚类 IS-PCR 的 VIII 的组被分配。SDS 页整个房间的蛋白质模式类似于 IS-PCR 指纹的那些。丰满的酸甲基酉旨(名声) 的部件和内容被用来区分代表性的紧张(Ls13， Ls8， BL1， BL12， HL6， Ls4 ) 从组我组织 VI。六代表性的紧张在细胞的丰满的酸甲基酉旨的内容和部件显示出重要差别。定序分析的 16S rDNA 证明那组拉紧我位于到 VII Enterobacteraceae (γ-proteobacteria ) 。组的紧张我和组 II 是仔细与 Klebsiella sp 有关；组 II 的种类 Ls8 是有点远的离开 Pantoea (与 Pantoea agglomerans 的相同水平 96%) 的类，它可以在 Enterobacteraceae 代表新种或类；组 IV 和 V 的种类属于不同 Enterobacter sp；种类 Ls4 和 L 9 代表组 VI 与 98% 顺序类似接近了 Citrobacter amalonaticus；组 VII 的种类 Ls15 与 Pantoea sp 显示出 98% 顺序身份；组 VIII 的种类被分到类 Ideonella (β-proteobacteria ) 。基于上述结果，从 O 孤立的 endophytic diazotrophs。rufipogon 证明大差异和一些 diazotrophs 与 42.52 μmol/mL · h C ₂ H ₄ 。到米饭测试的接种显示孤立的 endophytic diazotrophs 显著地支持了米饭生长。

英文摘要：

Diversity and nitrogenase activity of endophytic diazotrophs colonized in the wild rice Oryza rufipogon Griff grown in Boluo, Huilai County in Guangdong Province and Lingshui County in Hainan Province were studied. Thirty-seven isolates obtained from Oryza rufipogon were identified as putative endophytic nitrogen-fixing bacteria by ARA （acetylene reduction assay） test and further confirmed by PCR amplification of nifH gene fragments. All obtained strains have ARA activity and the same sized nifH gene fragments. Above the similarity level of 80%, the obtained isolates were assigned as Group Ⅰ to Ⅷ by the clustering of IS-PCR fingerprints. The SDS-PAGE whole-cell protein patterns were similar to those of IS-PCR fingerprints. Components and contents of fatty acid methyl esters （FAMEs） were used to differentiate the representative strains （Ls13, Ls8, BL1, BL12, HL6, Ls4） from Group Ⅰ to Group Ⅵ. The six representative strains showed significant difference in contents and components of cellular fatty acid methyl ester. 16S rDNA sequencing analysis showed that strains of Group Ⅰ to Ⅶ were located in Enterobacteraceae （y-proteobacteria）. Strains of Group Ⅰ and Group Ⅱ were closely related to Klebsiella sp.; Strain Ls8 of Group Ⅱ was a little far away from the genus of Pantoea （homology level 96% with Pantoea agglomerans）, which may represent a new species or genus in Enterobacteraceae; Strains of Groups Ⅳ and Ⅴ belonged to different Enterobacter sp.; Strain Ls4 and Ls 9 representing Group Ⅵ were close to Citrobacter amalonaticus with 98% sequence similarity; Strain Ls15 of Group Ⅶ showed 98% sequence identity with Pantoea sp.; Strains of Group Ⅷ were assigned to the genus Ideonella （β-proteobacteria）. Based on the above results, endophytic diazotrophs isolated from O. rufipogon showed great diversity and some diazotrophs showed high nitrogenase activity with 42.52 μmol/mL. h C2H4. Inoculation to rice tests indicated that the isolated endophytic diazotrophs significan