中文摘要：

目的：建立红外光谱快速鉴别羌活和宽叶羌活两种基原植物药材的方法。方法：收集了8份羌活和4份宽叶羌活根茎和根药材,用傅里叶变换红外光谱技术测定4 000～400 cm^（-1）范围内的一维红外光谱和二维相关红外光谱,计算二阶导数光谱,定量测定峰的强度,并进行光谱解析、主成分分析。结果：羌活与宽叶羌活药材一维红外光谱的特征峰不同。羌活在1 739（吸光度为0.39）、1 428（0.46）、1 076（0.91）、863（0.03）、764（0.08）cm^（-1）处有明显吸收峰,而宽叶羌活在此处的峰不明显（强度小于阈值0.01）;宽叶羌活在1 719（0.34）、1 607（0.50）、1 444（0.41）、823（0.06）、775（0.07）cm～（-1）处的吸收峰明显,而羌活在此处的峰不明显。在模式识别-主成分分析聚类图中,羌活与宽叶羌活分布于不同区域。二阶导数谱中两者主要共有峰的强度明显不同,羌活在1 747、1 468、1 159、1 078和988 cm～（-1）的峰强度明显高于宽叶羌活。而宽叶羌活在1 627、1 605、1 568、1 512和1 269 cm^（-1）峰强度明显高于羌活。二维相关红外光谱中两者自动峰的数量、位置及其相互关系不同。羌活样品在850～1 500 cm^（-1）范围内有13个自动峰,而宽叶羌活为9个自动峰。结论：根据一维、二阶导数和二维相关红外光谱,羌活与宽叶羌活两种植物的药材可快速鉴别。峰强度定量比较、主成分分析、聚类分析增加了鉴别的客观性和准确性。

英文摘要：

Objective：To develop a method to rapidly identify two medicinal raw materials which came from the rhizome and root of Notopterygium incisum and Notopterygium franchetii by Fourier Transform Infrared Spectroscopy（FTIR）. Methods ：Eight Notopterygium incisum samples and four Notopterygiumfranchetii samples were collected from different cultivation areas in Sichuan of China. The one- dimensional infrared（1D-FTIR） spectra and two-dimensional correlation infrared（2D-FTIR）spectra of these herbal samples were detec- ted in the range of 4 000 - 400 cm-1. Their second derivative infrared （SD-FTIR）spectra were also calculated. The intensities of peaks on the 1D-FTIR, 2D-FFIR and SD-FrIR spectra were quantified. The whole spectrum profiles, characteristic peaks between the two herbs were further compared and analyzed by spectral analysis and principal component analysis （PCA）method. Results：The 1D-FTIR spectra between the two herbs were different. The peaks at 1 739 （ Absorbance was 0. 39 ）, 1 428 （0. 46）, 1 076 （0. 91 ） ,863 （0. 03 ） and 764 （0.08）cm -~ were obvious in Notopterygium incisum, while they were not observed in Notopterygiumfranchetii. Meanwhile, the peaks at 1 719（0. 34）, 1 607（0. 50） ,1 444（0. 41 ） ,823 （0. 06）and 775（0.07）cm-1 were obvious in Notopterygiumfranchetii,but they were not found in Notopterygium incisum. In the PCA dendrogram ,Notopterygium incisum and Notopterygiumfranchetii were distributed in dif- ferent areas. Comparing the SD-FTIR spectra found that their spectra were obviously different. The peaks located at 1 747,1 468,1 159, 1 078 and 988 cm- 1 in Notopterygium incisum were significantly stronger than those in Notopterygiumfranchetii, while the peaks located at 1 627,1 605,1 568,1 512 and 1 269 cm-1 in Notopteryglum franchetii were significantly stronger than those in Notopterygium inci- sum. Moreover, the number, position and interrelation of auto-peaks on the 2D-FTIR spectra were different between the two herbs. Therewere 1