中文摘要：

针对结核杆菌CFP10基因序列设计并合成了1对引物，以构建的含有该引物扩增序列的重组质粒作为阳性标准品，建立了检测CFP10基因的SYBRGreen I real-timePCR方法。检测结果显示，该方法线性关系好，标准曲线的相关系数达到0．997，对初始模板的检出下限为1×10^1copies／μL，比常PCR方法高100倍。表明建立的real—timePCR检测方法灵敏度高、特异性强、重复性好，可以用于结核杆菌CFP0的病原检测及定量分析。

英文摘要：

According to the sequence of the Mycobacterium tuberculosis CFP10 gene to established a SYBR GreenIreal-time PCR method that could detect Mycobacterium tuberculosis CFP10 gene. Designed and synthesized a pair of CFP10 gene primer to build positive standard sample that containing the sequence of the recombinant plasmid. Test results showed that a good linear relationship of this method, correlation coefficient of this method reached to 0.997, the lowest detection threshold of this method was ×10^1copies/μL that higherl00 times than the conventional PCR method. The method has high sensitivity and strong specific and good repeatability that can be used to detect the pathogen of Mycobacterium tuberculosis and quantitative analysis.