中文摘要：

目的评估γ干扰素释放试验（IGRA）检测颈部淋巴结细胞悬液对颈部淋巴结核的辅助诊断价值。方法将2015年1月至2016年5月在新疆维吾尔自治区人民医院收治的48例颈部淋巴结肿大患者采用复合诊断标准分为颈部淋巴结结核组（21例），非颈部淋巴结结核组（27例），对48例患者的外周血和颈部淋巴结细胞悬液进行γ干扰素释放试验检测，运用SPSS19．0软件进行数据分析，两组间斑点形成细胞（SFCs）个数的比较采用Mann-Whitney U检验，样本间率的比较采用x^2检验，以P〈0．05为差异具有统计学意义。结果IGRA检测颈部淋巴结细胞悬液和外周血的敏感度分别为90．48％（19／21）、95．24%（20／21），差异无统计学意义（x^2=0．00，P〉0．05），颈部淋巴结细胞悬液和外周血的特异度分别为70．37％（19／27）、92．59％（25／27），差异有统计学意义（x^2=4．43，P〈0．05）。颈部淋巴结结核组中IGRA检测外周血和颈部淋巴结细胞悬液的SFCs中位数及上下四分位数EM（Q1，Q）]分别为109（52，186）个、68（30，156）个，不同标本间检测的SFCs中位数差异无统计学意义（U=175．00，P〉0．05）。非颈部淋巴结结核组中IGRA检测外周血和颈部淋巴结细胞悬液的SFCsM（Q1，Q3）分别为0（0，18）个、0（0，3）个，不同标本间检测的SFCs中位数差异无统计学意义（U=271．00，P〉0．05）。颈部淋巴结结核组的淋巴结细胞悬液SFCs明显高于非颈部淋巴结结核组的淋巴结细胞悬液SFCs，差异有统计学意义（U=45．00，P〈0．05）。结论γ干扰素释放试验检测颈部淋巴结细胞悬液具有可行性，对颈部淋巴结结核的辅助诊断有一定的价值。

英文摘要：

Objective The aim of the study was to evaluate the value of cervical lymph node cells suspensions performed with interferon-gamma release assays （IGRA） for diagnosing cervical tuberculous lymphadenitis （CTL）. Methods Of the 48 patients with cervical lymphadenitis, 21 were confirmed as CTL, and 27 were diagnosed as non-CTL. IGRA using peripheral blood mononuelear cells （PBMCs） and cervical lymph node cell suspensions were performed to examine the IFN-7 response to the ESAT-6 and CFP-10 antigens. Spot forming cells （SFCs） in diffe- rent groups were compared using the Mann-Whitney U test. Categorical variables were compared using the Pearson Chi-squared test. The criterion for statistical significance was P〈0. 05. Results The IGRA sensitivity of Cervical lymph node cell suspensions and PBMC was 90.48 % （19/21） and 95.24 % （20/21）, respectively. The specificity of cervical lymph node cell suspensions and PBMC was 70. 37 % （19/27） and 92.59 % （25/27）, respectively. There was no significant difference of between sensitivity peripheral blood mononuclear cells and cervical lymph node cell suspensions （x^2= 0. 00, P〉0.05）. The specificity of cervical lymph node cell suspensions IGRA was significantly higher than that of peripheral blood mononuelear cells （x^2 = 4.43, P〈0. 05）. In CTL group, SFCs median of PBMC and cervical lymph node cell suspensions performed IGRA were 109 （52,186） and 68 （30,156）, respectively. There was no statistical difference in the SFCs median of PBMC and cervical lymph node cell suspensions （U= 175.00, P〉0.05）. In non-CTL group, SFCs median of PBMC and cervical lymph node cell suspensions performed IGRA were 0 （0, 18） and 0 （0,3）, respectively. There was no statistical difference in the SFCs median of PBMC and cervical lymph node cell suspensions. However, the SFCs of cervical lymph node cell suspensions in CTL group was significantly higher than that in non-CTL group （U= 45.00, P〈0. 05）. Conclusion Cervical lymph node cells