中文摘要：

目的：验证经亲和淘选噬菌体展示随机十五肽库获得的与ICAM-1高亲和力结合的目的肽的生物功能。方法：在动物体外用ELISA法检验目的肽与ICAM-1的结合。在动物体内，先荧光标记目的肽，后将目的肽注射人小鼠炎症模型体内，肾脏组织冰冻切片定位荧光物质。结果：ELISA法验证目的肽与人ICAM-1呈浓度依赖性结合。在动物体内，重新荧光标记的目的肽具有向高表达ICAM-1的炎症部位特异性聚集的功能。结论：此目的肽具有与组织中高表达的ICAM-1特异性结合的生物功能，说明此目的肽可尝试作为以ICAM-1为靶的“肽导向药物”的前导肽。

英文摘要：

Objection：Verify the biological functions of ICAM-1-binding peptide which had been screen from phage-displayed random 15-peptide library. Methods：in v/tro,to prove the target peptide conbines with ICAM-1 by ELISA analysis. In vivo, the target peptide was labeled with fluorescence and was injected in mouse inflammatory model bodys to orientate fluorescence by freezing tissues slices. Result：The target peptide combines with ICAM-1 related with concentration. The target peptide could accumulate toward ICAM-1 in vivo. Conclusion：The target peptide can conbine with ICAM-1 specially. It suggested that the target peptide might be used as the guiding peptide of ＂peptide guided drug＂ to inflammatory site with ICAM-1 expression in the future.