中文摘要：

本研究分别从广西的地方肉鸡及河北、辽宁的蛋鸡中分离到了3株禽白血病病毒。剖检疑似发病鸡只，采集病变的肝脏、脾脏组织，经过RT-PCR检测确定为ALV感染。将病变组织接种CEF细胞，连续传代2次，将细胞上清接种DF-1细胞，p27抗原检测为阳性。同时提取病毒基因组，用H5／AD1和H5／H7两对特异性引物进行PCR扩增，结果3株为ALV-J亚型。进一步设计ALV-J亚型gp85特异性引物进行PCR扩增并测序。结果确认分离到的3株病毒为ALV-J亚型，其gp85序列与标准毒株HPRS-103同源性为96％。同时，用p27单抗进行间接免疫荧光试验，测定毒力。综上所述，我们从广西地方肉鸡中分离到1株J亚型禽白血病病毒，从河北及辽宁的蛋鸡中分离到2株J亚型禽白血病病毒。

英文摘要：

In this study, we obtained three strains of ALV from the native meat type Chickens in Guangxi Province, layer Chickens in Hebei and Liaoning Provinces. We collected the livers and spleens of the chickens and confirmed they were infected by ALV using RT-PCR. The materials were inoculated to CEF and went through two passages. The supernatant was used to inoculate the DF -1. The p27 antigen was detected by ELISA. At the meantime, we extracted the virus genome. The results of PCR using the primers of H5/AD1 and HS/H7 showed that three strains belong to ALV-J. One pair of primers were designed to amplify the gp85 gene which specifies J subtype by PCR. The results demonstrated that three strains belong to J subtype. Sequencing analysis revealed that the gp85 gene of the three strains of ALV-J shared 96G genetic identity with ALV strain HPRS-103. At the meantime, we confirmed the virulence of the three strains through indirect fluorescence antibody assay using the monoclonal antibody against p27 protein. As a result, we obtained one strain of ALV-J from the native meat type Chickens in Guangxi Province, two strains of ALV-J from layer Chickens in Hebei and Liaoning Provinces.