中文摘要：

构建博尔纳病病毒pEGFP-p24基因重组表达质粒。通过PCR方法扩增获得博尔纳病病毒p24基因的完整序列，特此片段定向克隆到pEGFP—N1载体多克隆位点区，筛选重组阳性菌株，提取重组质粒，利用PCR方法和核酸序列测定验证重组质粒构建的正确性。PCR及核酸序列测定证明博尔纳病病毒pEGFP-p24基因重组表达质粒构建成功。构建的重组质粒将为研究博尔纳病病毒p24基因在真核细胞中的功能和作用提供实验依据。

英文摘要：

Whole sequence of p24 gene of Borna disease virus （ BDV ） was obtained through PCR amplification and inserted this fragment cloned into polyclonal site domain of vector pEGFP-N1 to construct a plasmid expressing pEGFP-BDV p24 gene by screening the positive recombinant strain, then extract the recombinant plasmid and proved the validity of the successfully constructed recombinant plasmid with PCR and DNA sequencing. The constructed recombinant plasmid will provide experiment foundation to study the function and action of BDV p24 gene in eukaryotic cells.