通过活性追踪,对四合木的甲醇提取物活性成分进行了初步分离,使用快速制备色谱(combiflash companion)对甲醇提取物的氯仿萃取相进行了进一步分离,得到18个较大的馏分,同时以松材线虫(Bursa phelenchus xylophilus)为供试对象,对各馏分杀线虫活性作了初步测定,结果表明:氯仿萃取相F4馏分有较强的杀线虫活性,具有进一步研究的价值.
By using the tracing method the active components of the methanol extract from Tetraena rnongolica were separated, and 18 fractions further separated from the chloroform phase extracting from the methanol extract were obtained by using the combiflash companion, which was in relatively large amounts from different levels in the column. Taking Bursaphelenchus xylophilus as the species, the nematicidal activities of the 18 fractions was preliminarily determined. The results showed that the fraction F4 had an outstanding intensive nematicidal activity against the Bursaphelenchus xylophilus, which would be worthy of further study.