中文摘要：

目的建立激素耐药型原发性肾病综合征（SRNS）儿童与激素敏感型原发性肾病综合征（SSNS）儿童尿液全蛋白双向凝胶电泳（2-DE）图谱，分析差异的蛋白质位点，为研究原发性肾病综合征（INS）糖皮质激素耐药的机制提供依据。方法病例来源于湘雅二医院儿科住院部，收集时间为2005-01-2005-10，入选的INS患儿依据中华医学会儿科学分会肾脏病学组制定的诊断标准，分为3组，每组5例。利用2-DE技术分离SRNS与SSNS（治疗前、后）儿童尿液总蛋白质，Image Master 2D V3.01分析软件进行凝胶图像分析。结果获得了图像清晰、分辨率高的SRNS组、SSNS（治疗前）与SSNS（治疗后）组儿童尿液2-DE图谱，3组点数分别为（206±8）个、（204±5）个、（55±5）个；SRNS组与SSNS组（治疗前）尿液2-DE凝胶共筛选出差异蛋白质点66个；SRNS组与SSNS组（治疗后）尿液2-DE凝胶共筛选出差异蛋白质点33个。结论该研究成功建立了分辨率高且重复性较好的SRNS与SSNS儿童尿液全蛋白的2-DE图谱；通过组间尿液2-DE图谱分析，发现了一些可能与INS激素耐药或敏感有关的蛋白质位点，为进一步运用蛋白质组学技术寻找与INS激素耐药抑或激素敏感相关的蛋白质打下了基础。

英文摘要：

Objective To investigate a proteome profile of urine in children with steroid-resistant or -sensitive idiopathic nephrotic syndrome （INS） by two-dimensional polyacrylamide gel electrophoresis （2-DE） ,in order to search for the different proteins for the further study of steroid-resistant mechanisms in INS. Methods Collect urine samples from children with steroid- resistant INS （SSNS） ,steroid-sensitive INS （SRNS） before and after steroid therapy,then use 2-DE and Image Master 2D V3.01 software to analyze the different expression of proteins in 2-DE gels. Results Mean protein spots of the 2-DE gels were （ 206 ± 8 ）, （ 204 ± 5 ）, （55 ± 5 ） in children with SRNS, SSNS before or after steroid treatment. Sixty-six different protein spots were screened between the SRNS and SSNS group before steroid therapy. Thirty-three different protein spots were screened between SRNS and SSNS after steroid therapy. Conclusion A distinct and reproducible 2-DE profile of urine proteome is successfully established in children with steroid-resistant and steroid-sensitive INS. By the differentiated analysis ,the differences of urine proteome profiles are discovered between steroid- resistant and steroid-sensitive INS,and some of these special proteins may be related to the mechanism of steroid-resistant and steroid-sensitive in INS.