中文摘要：

AIM To investigate whether the proportions of acetylhistone-positive hepatocytes could be used as markersof deteriorating liver function.METHODS： In total, 611 cirrhotic cases from 3701patients who were diagnosed during the past 15years were screened, and 152 follow-up cases wereselected. Paraffin tissue microarray was preparedfor immunohistochemistry to examine acetyl-histoneexpression. The proportions of positive hepatocyteswere recorded, and their correlations to clinical andlaboratory indicators were analyzed statistically.RESULTS： The proportions of H2AK5ac＋, H3K9/K14ac＋and H3K27ac＋ hepatocytes gradually increased withdeteriorating liver function and with increasing levelsof serum markers of liver injury. In the follow-upcases, patients with 〉 70% H2AK5ac＋, H3K9/K14ac＋ orH3K27ac＋ hepatocytes had statistically lower survivalrates （P 〈 0.05）. Furthermore, 〉 70% H2AK5ac＋or H3K27ac＋ hepatocytes were strong independentpredictors of overall survival （P 〈 0.05）.CONCLUSION： The proportions of acetyl-histonepositivehepatocytes are closely associated with theliver function and prognosis of cirrhotic patients.

英文摘要：

AIM: To investigate whether the proportions of acetylhistone- positive hepatocytes could be used as markers of deteriorating liver function. METHODS: In total, 611 cirrhotic cases from 3701 patients who were diagnosed during the past 15 years were screened, and 152 follow-up cases were selected. Paraffin tissue microarray was prepared for immunohistochemistry to examine acetyl-histone expression. The proportions of positive hepatocytes were recorded, and their correlations to clinical and laboratory indicators were analyzed statistically. RESULTS: The proportions of H2AK5ac(+), H3K9/K14ac(+) and H3K27ac(+) hepatocytes gradually increased with deteriorating liver function and with increasing levels of serum markers of liver injury. In the follow-up cases, patients with > 70% H2AK5ac(+), H3K9/K14ac(+) or H3K27ac(+) hepatocytes had statistically lower survival rates (P < 0.05). Furthermore, > 70% H2AK5ac(+) or H3K27ac(+) hepatocytes were strong independent predictors of overall survival (P < 0.05). CONCLUSION: The proportions of acetyl-histonepositive hepatocytes are closely associated with the liver function and prognosis of cirrhotic patients.