中文摘要：

合成了4种不同形貌和尺寸的二水草酸钙（COD）晶体,分别为尺寸20μm的花状、10μm的十字形、2μm的四方双锥形和1μm的棒状COD晶体。采用X射线衍射、傅立叶变换红外光谱、扫描电子显微镜（SEM）和比表面测试仪对上述4个COD晶体的理化性质进行了表征。花状和十字形COD晶体都具有尖锐的棱角;棒状、四方双锥形COD都具有完整的（100）面。随着晶体尺寸的降低和晶体表面正电荷密度高的（100）面比例增大,其Zeta电位绝对值不断减小。不同形貌COD晶体均对非洲绿猴肾上皮细胞（Vero）具有毒性作用,其细胞毒性大小与多因素相关,包括晶体的尺寸、晶面、尖锐程度、比表面积以及Zeta电位等。本研究有助于进一步阐明尿微晶形貌差异影响肾结石形成的分子和细胞机制。

英文摘要：

Four kinds of calcium oxalate dihydrate （COD） crystals with different sizes and morphologies were synthesized, they were flower-like COD of 20 （jim, cross-shaped COD of 10 （jim, bipyramid COD of 2 （jim and rod-like COD crystal of 1 jjim, respectively. X-ray diffraction, Fourier transform infrared spectrometer, scanning electron microscope, and specific surface tester were used to characterize the physical and chemical properties of these four COD crystals. The results showed that flower-like and cross-shaped COD crystals have sharp edges and comers, rod-like and bipyramid COD crystals have complete （100） face. The absolute value of Zeta potential of COD crystals decreased with the decrease of crystal size and the increase of positively-charged （ 100） face of COD. The cytotoxicity of various COD crystals on African green monkey renal epithelial cells was closely correlated with the crystal size, crystal face, specific surface area, pore size, and Zeta potential of the crystals. This study can help to clarify the effect of crystal shape on stone formation mechanism at molecular and cellular level.