中文摘要：

背景与目的：表柔比星（epirubicin）是临床上治疗乳腺癌的一线化疗药物,脂质体多柔比星（liposome doxorubicin）是一种新型脂质体类药物,相比传统蒽环类药物可以降低心脏毒性和骨髓抑制。树突状细胞（dentric cell,DC）在肿瘤免疫中起到重要作用。本实验旨在探讨这2种药物对人乳腺癌细胞株Bcap37和MDA-MB-231,以及人树突状细胞生长的抑制作用,以评估2种制剂在乳腺癌治疗中的价值。方法：用不同浓度（0、0.25、0.5、1.0、2.0、4.0μg/ml）表柔比星和脂质体多柔比星分别作用于Bcap37、MDA-MB-231和树突状细胞,MTT法检测24、48和72h时对细胞生长的抑制率。结果：2种药物对肿瘤细胞和正常细胞均有抑制作用。与对Bcap37、MDA-MB-231的作用相比,脂质体多柔比星对树突状细胞的抑制作用较小（F=22.208,P〈0.01;F=20.534,P〈0.01）。脂质体药物对Bcap37的抑制作用强于MDA-MB-231（F=12.873,P〈0.01）。结论：恶性程度低的乳腺癌细胞对脂质体多柔比星的敏感性高,恶性程度高的细胞则化疗敏感性低。脂质体制剂较普通制剂对于正常树突状细胞的毒性小。

英文摘要：

Background and purpose： Epirubicin is one of the first line chemotherapy drugs in the treatment of breast cancer, and liposome doxorubicin is a new antitumor drug that has been reported to have less cardiotoxicity and myelosuppression compared to free doxorubicin. Dentritic cells （DC） play important roles in tumor immunity. Our experiment investigated the impacts of epirubicin and liposome doxorubicin on different human breast cancer cell lines and dentritic cells, and evaluated their roles in the treatment of breast cancer. Methods： Human breast cancer cell lines, Bcap37 and MDA-MB-231, along with human dentritic cells isolated and induced into maturation, were cultured with epirubicin and liposome adriamycin at different concentrations （0, 0.25, 0.5, 1.0, 2.0, 4.0, 10.0 μg/ml）, respectively. The inhibitory effects were detected by MTT method after 24, 48, 72 h. Results： Epirubicin and liposome adriamycin could inhibit the proliferation of Bcap37 cells, MDA-MB-231 cells, and human dentritic cells. Liposome adriamycin exhibited a lighter inhibition on dentritic cells than on human breast cancer cell lines （Bcap37 and MDA- MB-231） （F=22.208, P〈0.01; F=20.534,P〈0.O1）. Compared with MDA-MB-231 cells, the proliferation of Bcap37 cells was inhibited more effectively by liposome adriamycin. Conclusion： Breast cancer cell lines with lower degree of malignancy appear to be more sensitive to chemotherapy, as compared to those with higher degree. The toxicity of liposome adriamycin on dentritic cells is lower than epirubicin.