中文摘要：

目的建立卵巢癌HO-8910PM细胞胶原Ⅰ三维培养模型,对胶原Ⅰ基质中卵巢癌HO-8910PM细胞的形态、活力及功能进行研究,为体外卵巢癌的研究提供参考。方法透射电镜表征胶原Ⅰ结构特点,以胶原Ⅰ为支架材料,建立卵巢癌细胞体外三维培养模型;倒置显微镜观察卵巢癌细胞生长形态及增殖情况;钙黄绿素-AM染色观察胶原Ⅰ基质中卵巢癌细胞的活力;ELISA检测二维培养及三维培养中卵巢癌细胞血管内皮生长因子-A（VEGF-A）、表皮生长因子（EGF）和胰岛素样生长因子-1（IGF-1）的分泌。结果透射电镜下可见胶原Ⅰ呈交联网络状结构,胶原Ⅰ基质中卵巢癌细胞集聚成不规则团状,细胞在胶原Ⅰ中的活力较好,细胞与基质具有良好的生物相容性;与二维培养细胞比较,三维培养体系中VEGF-A、EGF和IGF-1的分泌量均较高,差异有统计学意义（P〈0.05或0.01）。结论胶原Ⅰ具有良好的结构与性能,适宜于卵巢癌细胞体外三维培养,并能较好地维持卵巢癌细胞的形态和功能。

英文摘要：

Objective To establish the three-dimensional（3D） cultivation model of ovarian cancer HO-8910 PM cell line using collagenⅠ as scaffolds and observe the cellular morphologies,vitalities and functions to provide references for the study of ovarian cancer in vitro. Methods TEM was used to be assess to representation of characteristics of collagen Ⅰformation. Relied on collagenⅠ as raw materials,it was established three-dimensional system model. The growthform and proliferation of ovarian carcinoma cells were observed by inverted microscope. The viabilities of the ovarian carcinoma cells in the collagen Ⅰ matrix were examined by calcein-AM staining;The expression of VEGF-A,EGF and IGF-1 in collagen Ⅰmatrix were inspected by Elisa assay. Results CollagenⅠ displayed a cross-linked network structure under transmission electron microscope Ⅰ. The ovarian carcinoma cells in the collagenⅠ matrix were irregular regiment massive structure and showed a good vitality in the collagen Ⅰ. It had a good biocompatibility between the cells and matrix. Compared to two-dimensional cultured cells,the secretion quantity of VEGF-A,EGF and IGF1 in the three-dimensional cultivation system was higher. The difference had statistical significance（P〈0.05）. Conclusion CollagenⅠ can support ovarian cancer three-dimensional culture adequately with good structure and functions,which is suitable for three-dimensional cultivation of ovarian carcinoma cells in vitro,maintaining the form and functions of ovarian cancer cells.