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中文摘要：
利用紫外-可见吸收光谱法和荧光光谱法研究了抗癌药物硫鸟嘌呤（6-TG）与七元瓜环（Q[7]）及牛血清白蛋白（BSA）的相互作用.结果表明,6-TG与Q[7]及BSA可形成三元复合物,且6-TG与Q[7]及BSA均可形成1∶1的超分子配合物,6-TG能引起BSA的荧光猝灭,猝灭机制为静态猝灭.此外,还用同步荧光法和三维荧光法考察了6-TG对BSA构象的影响,结果表明6-TG的加入使BSA的构象发生了变化,而同步荧光光谱结果表明结合位点更接近于色氨酸.
英文摘要：
The aim of the present study was to investigate the interaction of 6-thioguanine（6-TG） with cucurbit[7]uril（Q[7]） and bovine serum albumin（BSA） in an acetate buffer solution by ultraviolet absorption spectroscopy,fluorescence emission spectroscopy and synchronous fluorescence spectra,as well as and three-dimensional fluorescence spectra.It was indicated from UV-Vis spectra that 6-TG-Q[7] binary complex formed.The fluorescence quenching of BSA by 6-TG was a result of the formation of 6-TG-BSA complex,and the quenching mechanism of BSA by 6-TG was static quenching mechanism.Moreover,effect of 6-TG on BSA conformation was discussed by the studies of synchronous and three-dimensional fluorescence spectra.In addition,a specific binding interaction took place between the 6-TG and Q[7] in the presence of BSA.
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