中文摘要：

在营养液水培条件下,分析叶片预喷施5%（V/V）甲醇对50μmol.L-1的AlCl3胁迫下黑大豆叶和根中H2O2和MDA含量以及抗氧化酶SOD、POD和CAT活性的影响,为进一步研究甲醇及抗氧化酶在响应铝胁迫应答中的调控机制奠定基础。结果显示：（1）在铝胁迫下,随着铝处理浓度的升高根尖吸收的铝越多,且根的生长被抑制越严重。（2）铝胁迫能诱导黑大豆叶和根中可溶性总蛋白、H2O2和MDA含量的增加,POD和CAT活性增强,但SOD活性变化不明显。（3）当叶片喷施5%甲醇预处理后再进行50μmol.L-1 AlCl3胁迫,随着处理时间增加黑大豆叶和根中可溶性总蛋白含量及POD和CAT活性显著增加,而H2O2和MDA含量显著下降,SOD活性变化亦不显著。研究结果表明,在铝胁迫下叶片喷施5%甲醇能够增强黑大豆叶和根中POD和CAT活性,降低H2O2和MDA的积累,这可能是甲醇通过抗氧化酶参与铝胁迫应答的一种重要机制。

英文摘要：

In a nutrient solution hydroponic conditions,effect of foliar pre-application of 5%（V/V） methanol on the contents of H2O2 and MDA,and antioxidant enzyme activities（SOD,POD and CAT） in leaf and root of black soybean [Glycine max（L.） Merrill] were investigated under 50 μmol·L-1 Al3＋ stress,and to lay the foundation for further studying on the regulatory mechanism of methanol and antioxidant enzymes response to Al stress.The results showed that：（1）Under Al stress,the absorption amount of aluminum from root tips was more with increasing Al3＋ treatment concentration,and the root growth was more seriously inhibited.（2）Al stress could induce the increasing of the contents of soluble protein,H2O2 and MDA,POD and CAT activities in leaf and root of black soybean,but SOD activity was no significantly change.（3）After foliar pre-application of 5% methanol treatment,under 50 μmol·L-1 Al3＋ stress the contents of soluble protein,POD and CAT activities were significantly ascend with increasing treatment time,while the contents of H2O2 and MDA in leaf and root were declined,and SOD activity was also no change.The study results have shown that foliar application of 5% methanol could significantly increase antioxidant enzyme activities（CAT and POD）,and decline the accumulation of H2O2 and MDA in leaf and root of black soybean under Al stress.This may be an important mechanism for methanol taking part in response to Al stress by regulating antioxidant enzyme activities.