中文摘要：

应用重组维甲酸X受体（RXR）基因酵母筛选南方某污水处理厂进水、不同工艺出水的维甲酸干扰活性;结合大鼠肝均浆（S9）体外代谢方法,检测样品间接维甲酸干扰活性.结果表明：进水、出水样品均不能诱导RXR介导酶活性,但在高富集倍数下,样品与空白对照相比显著抑制9-顺维甲酸诱导酶活性,表现出维甲酸拮抗效应;在添加S9体外代谢活化后,所有样品均检出类维甲酸干扰活性,部分样品还检测出抗维甲酸活性,表明城市污水样品中存在大量具有类/抗维甲酸干扰活性的化合物.研究结果证实,现行处理工艺对类/抗维甲酸干扰物有一定的去除效果;重组基因酵母结合S9体外代谢活化体系可用于水环境样品类/抗维甲酸干扰效应的快速筛选.

英文摘要：

The recombinant retinoid X receptor（RXR） gene yeast was conducted to assess the RXR ant/agonistic activities of effluents collected from wastewater treatment plant（WWTP） at a city in south China.Also,a metabolic activation method was introduced based on rat liver S9 for screening indirect RXR ant/agonists activity.The results indicated all of the samples including influent and effluent couldn＇t induce the enzyme activity.But,compared with blank control,all samples with higher concentration obviously inhibited the enzyme activity induced by 9-cis-retinoic acid.After metablization,the agonistic activation were found in all samples,and the antagonistic activation were also found in some samples,suggesting that there are a lot of chemical compound with RXR ant/agonists in wastewater.All of the results demonstrate that the present treatment processes could remove some RXR ant/agonists and suggest that the combined method of recombinant RXR gene yeast assay with S9 metabolic activation can be used for screening the RXR ant/agonistic activity of environmental samples.