凝集素,半乳糖绑定,可溶 1 (LGALS1 ) 基因编码 galectin-1,在 myoblast 增长和区别期间起一个重要作用的不正常的能分泌的蛋白质。在这研究,猪的 LGALS1 基因从猪肌肉被克隆并且描绘。预言的蛋白质顺序与它的哺乳动物的对应物分享了高身份。反向的抄写聚合酶链反应表明猪的 LGALS1 在 33 白天被表示性交以后(dpc ) 并且 65 dpc 在一相对高级,然后在胎儿的骨胳的肌肉开发期间减少了到 90 dpc,建议 galectin-1 是一个有势力因素在 myofibers 的形成含有。LGALS1 被发现广泛地在所有纸巾表示了,短暂转染显示 galectin-1 在细胞质和原子核定位两个。Genomic 序列和分析在近似 1.279-1.529 kb 预言了一个倡导者区域,但是双酶的记者试金显示它举办小倡导者活动。
Lectin, galactose-binding, soluble 1 (LGALS1) gene encodes galectin-1, an atypical secretory protein that plays an important role during myoblast proliferation and differentiation. In this study, the porcine LGALS1 gene was cloned and characterized from pig musde. The predicted protein sequence shared a high identity with its mammalian counterparts. Reverse transcription-polymerase chain reaction revealed that porcine LGALS1 was expressed at 33 day post-coitus (dpc) and 65 dpc at a relatively high level, and then decreased to 90 dpc during fetal skeletal muscle development, suggesting that galectin-1 is a potent factor implicated in the formation of m yofibers. LGALS1 was found widely expressed in all tissues and transient transfection indicated that galectin-1 locates both in cytoplasm and nucleus. Genomic sequences and analysis predicted a promoter region at approximately 1.279-1.529 kb, but dualluciferase reporter assay indicated that it has little promoter activity.