中文摘要：

目的：研究围生期双酚A（Bisphenol A,BPA）暴露对SD大鼠子代生殖系统的影响及雌激素受体（Estrogen Receptor,ER）表达的改变。方法：经灌胃给予围生期孕鼠0、5、50、500mg/（kg.d）的BPA,观察雌性子代生殖器官重量、病理学改变、子宫ERα、β及磷酸化ERK（p-ERK）与总ERK（T-ERK）蛋白表达的改变,并通过子宫增重实验检测子宫对雌二醇（E2）敏感性的改变。结果：500mg/kg BPA可引发亲代中毒症状及子代体重下降（P〈0.05）,50mg/kg BPA可明显增加雌性子代体重及子宫重量,BPA各剂量组均可使子宫内膜增厚及相对比例增加,子宫增重和子宫相对增殖率增加,并提高ERα与p-ERK蛋白表达,而对ERβ无影响。结论：BPA围生期暴露可引起子代子宫组织ER蛋白表达变化,并可提高子宫对E2的敏感性。

英文摘要：

OBJECTIVE：To explore the effect of perinatal exposure to bisophenol A（BPA）on reproduction and development of the female offspring of SD rats and the changes of uterine estrogen receptor（ER）expression.METHODS：Pregnant Sprague-Dawley rats received by gavage 0,5,50 or 500 mg/（kg·d）of BPA from gestation day（PND）6 to postnatal day（PND）21.Uterus and ovarians were weighed and prepared for histological evaluation.The expression of ERα,ERβ,phosphorylated ERK（p-ERK）and total ERK（T-ERK）in uterus were measured.The change of uterine sensitivity to estradiol（E2）was investigated by the modified uterotrophic assay.RESULTS：The toxic symptoms in parental rats and decrease in body weight in female offsprings were observed in 500 mg/（kg·BW）group,but there was an increase in body weight and uterine weight in the 50 mg/（kg·BW）group.No pathological changes were found in BPA-treated group,the thickness of endometrial layer and the ratio to uterus was increased in all dosage groups compared with the control.The protein expressions of ERα and p-ERK but not ERβ in uterus were elevated in all dosage groups.The uterine sensitivity to E2 was increased in all BPA-treated groups.CONCLUSION：Perinatal exposure to Bisphenol A could induce the changes of ER expression in the uterus of female offspring rats and change the uterine physical structure by probably activating the ERK pathway.