中文摘要：

目的：探讨纳米二氧化硅（SiO2）颗粒对HL-7702细胞的毒性作用和细胞缝隙连接通讯（GJIC）的影响,为纳米SiO2体内外毒性的预测和安全性应用提供实验依据。方法：透射电镜（TEM）观察2种SiO2颗粒的粒径、分散性和形状;动态光散射法（DLS）检测SiO2颗粒在高纯水和培养液中的粒度分布;MTT法检测细胞存活率;乳酸脱氢酶（LDH）活力实验检测细胞膜完整性;划痕染料示踪技术检测GJIC。结果：透射电镜,2种SiO2颗粒呈圆形,大小均一,分散性良好,2种颗粒的粒径分别为（447.60±20.78）和（67.42±5.69）nm,分别为亚微米级和纳米级颗粒。动态光散射法,2种颗粒在高纯水和RPMI-1640培养液中的水合粒径分别为（684.37±18.76）、（697.02±19.57）nm和（128.31±7.64）、（133.74±8.97）nm,颗粒均未发生聚集,分散性良好。MTT法,2种SiO2颗粒作用细胞24h后,同一粒径的颗粒,随着浓度的增加,细胞存活率下降;同一浓度下,纳米颗粒比亚微米颗粒的毒性大。LDH活力实验,当作用细胞24h后,2种SiO2颗粒均能够损伤细胞膜,同一浓度下,纳米SiO2颗粒比亚微米颗粒对细胞膜的损伤能力大;同一粒径的颗粒,随着作用浓度的增加,细胞膜的损伤程度加重。划痕染料示踪实验,纳米SiO2颗粒可抑制GJIC,并且随着作用浓度的增加,抑制作用增强;而在相同浓度下,纳米SiO2颗粒比亚微米颗粒对GJIC的抑制作用更明显。结论：纳米SiO2颗粒能够对HL-7702细胞产生毒性作用,且抑制GJIC。

英文摘要：

Objective To study the cytotoxicity and effect on gap junction intracellular communication（GJIC） of SiO 2 nanoparticles in HL-7702cells,and to provide experimental basis for toxicity assessment and the security applications of SiO 2 nanoparticles.Methods Transmission electron microscope（TEM） was used to characterize two kinds of SiO 2 nanoparticles,verifying their size,dispersion and shape;dynamic light scattering（DLS） method was used to analyze the water dispersion and culture medium dispersion of the SiO 2 nanoparticles;MTT assay was carried out to examine the cytotoxicities of the two sizes SiO 2 nanoparticles on the cells;lactate dehydrogenase （LDH） release assay was performed to examine the integrity nano of the cell membrane;Scrape-loading and dye transfer assay was performed to examine the effect of SiO2nanoparticles on GJIC.Results Based on the result of TEM,two kinds of SiO 2 nanoparticles were spherically shaped,uniformly sized and sporadically dispersed;the statistical analysis results showed the diameters of the two nanoparticles were（447.60±20.78） nm and（67.42± 5.69） nm,respectively,thus they could be categorized as submicron scale and nano scale.The DLS method results manifested that the hydration nanoparticle sizes of the two SiO 2 nanoparticles were（684.37±18.76） nm,（128.31±7.64） nm in high purity water and（697.02±19.57） nm,（133.74±8.97） nm in RPMI-1640solution,all the two nanoparticles were well dispersed without aggregation.MTT assay indicated that 24hafter treatment of SiO 2 nanoparticles,the cell viabilities were affected by both the size and the dose of the SiO2 nanoparticles;the higher the dose was,the less viability the cells exhibited.Moreover,the nano scale particles inflicted more damage to the cells.LDH release assay indicated that the SiO2 particles could also damage the cell membrane in a dosedependent and size-dependent way.Scrape-loading and dye transfer assay indicated that the nano scale particles could cause GJIC inhibition in a dose-