中文摘要：

目的：探讨金黄色葡萄球菌脂磷壁酸（LTA-sa）促进破骨细胞分化的分子机制。方法：以浓度为200 ng/m L的LTA-sa作用于Raw264.7细胞,分别作用0、5、10、20、40、60 min及0、1、2、3 d后,用Western blot检测破骨细胞分化过程中相关信号通路的蛋白表达水平;并以100、200及400 ng/m L的LTA-sa及磷酸盐缓冲液作用于Raw264.7细胞,于作用后1、2、3 d用酶联免疫吸附法（ELISA）检测TNF-α、IL-1α及IL-6的表达情况。结果：（1）Western blot显示,在LTA-sa的作用下,IκB-α在5、10 min时降低,而p-NFκB在10 min时表达明显增高;此外,NFATc1在LTA-sa作用后第2及第3天表达逐渐增高,上述结果经统计分析,差异均有统计学意义（均P〈0.001）。（2）ELISA检测发现,IL-6在第2、3天表达增高,并随LTA-sa浓度的增加及作用时间的延长,其表达逐渐增多,经统计分析差异有统计学意义（均P〈0.001）。结论：LTA-sa通过NF-κB信号通路及IL-6的分泌促进破骨细胞分化。

英文摘要：

Objective To investigate the molecular mechanism of osteoclast differentiation induced by staphylococcal lipoteichoic acid（LTA-sa）. Methods Raw264.7 cells were treated with LTA-sa in a concentration of 200 ng / m L for 0, 5, 10, 20, 40, 60 min and 0, 1, 2, 3 days respectively, and the proteins in signaling pathways associated with osteoclast differentiation were measured with western blot. In addition, Raw264.7 cells were treated with different concentrations of LTA-sa（100, 200 and 400 ng / m L） and PBS for 0, 1, 2, 3 days,the expression of TNF-α, IL-1α and IL-6 was detected with Enzyme linked immunosorbent assay（ELISA）.Results（1）Western blot showed that, under stimulation of LTA-sa, IκB-α decreased at 5 min and 10 min,while the phosphorylation of nuclear factor κB increased at 10 min. In addition, NFATc1 increased in 2 and3 days gradually. The above results were statistically analyzed, and the difference was significant in statistics（P〈0.001）.（2）ELISA showed that the expression of IL-6 increased in 2 and 3 days along with the increasing concentration and prolonging stimulation time of LTA-sa. Data were statistically analyzed, the difference was significant in statistics（P〈0.001）. Conclusion LTA-sa promotes osteoclast differentiation through the NF-κB signaling pathway and the secretion of IL-6.