中文摘要：

这研究是调查 phospholipase D (PLD ) 和 lipoxygenase (哈鱼) 的回答到在 postharvest 黄瓜的机械伤害(Cucumis sativus L。cv。Biyu-2 ) 水果。联系膜的 Ca2+ 内容， PLD 和哈鱼的活动和基因表达式，和 phosphatidylcholine (PC ) 的内容， phosphatidylinositol (PI ) ，和 phosphatidic 酸(PA ) 在跟随机械伤害的黄瓜水果被决定。结果证明 PLD 和哈鱼活动与是在伤害之上的 upregulated 的 PLD 和哈鱼 mRNAs 增加了，当联系膜的 Ca2+ 内容减少了时。随 PLD 和哈鱼活动的增加伴随， PA 的累积和 PC 和 PI 的损失在所有水果被观察，但是有度在之间的差别伤害并且控制水果。结果建议 PLD 和哈鱼可能是在参予机械伤害损害的 postharvest 黄瓜水果的 phospholipids 的主要 hydrolytic 酶。PLD 和哈鱼的激活可能是基因表示的结果，它能被在收到伤害的信号之上从膜流动到细胞质的 Ca2+ 刺激。关键词黄瓜 - 机械伤害 - Phospholipase D - Lipoxygenase - Phospholipid CLC 数字 S642.2 工程(号码 30771513 ) 由中国的国家自然科学基础支持了

英文摘要：

This study was to investigate the responses of phospholipase D（PLD） and lipoxygenase（LOX） to mechanical wounding in postharvest cucumber（Cucumis sativus L.cv.Biyu-2） fruits.Membrane-associated Ca2＋ content,activities and gene expression of PLD and LOX,and contents of phosphatidylcholine（PC）,phosphatidylinositol（PI）,and phosphatidic acid（PA） were determined in cucumber fruits following mechanical wounding.Results show that PLD and LOX activities increased with the PLD and LOX mRNAs which are upregulated upon wounding,while membrane-associated Ca^2＋ content decreased.Accompanying with the increase of PLD and LOX activities,accumulation of PA and losses of PC and PI were observed in all fruits,but there were differences of degrees between wounded and control fruits.Results suggest that PLD and LOX might be the main hydrolytic enzymes of phospholipids in postharvest cucumber fruits participating in the mechanical wounding injury.The activation of PLD and LOX might be the result of gene expression,which could be This study was to investigate the responses of phospholipase D（PLD） and lipoxygenase（LOX） to mechanical wounding in postharvest cucumber（Cucumis sativus L.cv.Biyu-2） fruits.Membrane-associated Ca^2＋ content,activities and gene expression of PLD and LOX,and contents of phosphatidylcholine（PC）,phosphatidylinositol（PI）,and phosphatidic acid（PA） were determined in cucumber fruits following mechanical wounding.Results show that PLD and LOX activities increased with the PLD and LOX mRNAs which are upregulated upon wounding,while membrane-associated Ca2＋ content decreased.Accompanying with the increase of PLD and LOX activities,accumulation of PA and losses of PC and PI were observed in all fruits,but there were differences of degrees between wounded and control fruits.Results suggest that PLD and LOX might be the main hydrolytic enzymes of phospholipids in postharvest cucumber fruits participating in the mechanical wounding injury.The activation of PLD and LOX