中文摘要：

根据生物矿化策略,以不同质量浓度的Na2SeO3溶液作为硒源,利用大肠杆菌MG1655生物还原Na2SeO3制备纳米硒。研究Na2SeO3质量浓度对大肠杆菌生长曲线的影响,用氢化物原子荧光光谱法（HG-AFS）测定Na2SeO3的还原效率,进一步利用扫描电子显微镜（SEM）、X线衍射仪（XRD）和傅里叶红外光谱（FT-IR）等仪器对产物进行了相关表征。结果表明：大肠杆菌MG1655能将Na2SeO3大量矿化为粒径基本在100-250 nm左右的红色单质纳米硒颗粒,且对各质量浓度下的Na2SeO3的还原率均在50%以上。

英文摘要：

The reduct ion with Escherichia coli MG 1655 of sodium selenite was used to prepare nano-selenium wi th d iffe re nt mass concentrations of sodium selenite as selenium source artificially . The influence of sodium selenite mass concentration on the growth curve of Escherichia coli MG 1655 was investigated. The reduct ion ef f icie ncy of sodium selenite was determined by hydride generation atomic fluorescence spectrometry （ H G - A F S ）,and the structure of the product was characterized by scanning electron microscope （ S EM ）, X - raydiffra ction （ XRD ） and F ourier transform infrared spectroscopy （F T - IR ）. Results indicated that sodium selenite could be biomineralized in abundance by Escherichia coli MG 1655 to nano-selenium particles in red simple substance w i th th e ir diameters mainly 100 -250 nm . Moreover, al l the reduct ion rates of various mass concentrations of sodium selenite solution were over 50%.