中文摘要：

目的探讨胃癌细胞中长链非编码RNA SNHG5与miR-26b的相互作用。方法实时定量PCR检测SNHG5在GES-1胃黏膜正常上皮细胞与胃癌细胞中的表达差异,通过瞬时转染SNHG5过表达质粒,Taq Man定量PCR检测MGC-803细胞中miR-26b及pri-miR-26b的表达变化,同时瞬时转染miR-26b mimics检测其对SNHG5表达的影响。结果 SNHG5在胃癌细胞中的表达明显高于胃黏膜正常上皮细胞GES-1（P〈0.05）,SNHG5能够抑制miR-26b与pri-miR-26b的表达（P〈0.01）,但miR-26b不能对SNHG5的表达产生影响。结论 SNHG5可抑制miR-26b的表达,具体作用机制还需要进一步分析。

英文摘要：

Objective To explore the interaction between miR-26 b and lncRNA SNHG5 in gastric cancer. Methods Quantitative real-time PCR analyzed the differential expression of SNHG5 in normal gastric epithelial cell GES-1 compared with it in gastric cancer cell. Transient transfected MGC-803 cells with SNHG5 overexpressed plasmid and detected the expression level of miR-26 b and pri-miR-26 b by Taq Man PCR analysis. Meanwhile,transient transfected MGC-803 cells with miR-26 b mimics to detect the expression change of SNHG5. Results Expression level of SNHG5 was significantly higher in gastric cancer cells than in normal gastric epithelial cell GES-1（ P〈0. 05）. SNHG5 inhibited the expression of miR-26 b and pri-miR-26b（ P〈0. 01）,but miR-26 b could not influence the expression of SNHG5. Conclusions SNHG5 inhibites the expression of miR-26 b,but the mechanism need to be further clarified.