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Genetic analysis and gene mapping of a narrow leaf mutant in rice (Oryza sativa L.)
  • ISSN号:1001-6538
  • 期刊名称:科学通报(英文版)
  • 时间:0
  • 页码:752-758
  • 语言:中文
  • 分类:S511[农业科学—作物学]
  • 作者机构:[1]Crop Molecular Breeding Center, Institute of Crop and Nuclear Technology Utilization, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China
  • 相关基金:Supported by National High Technology Research and Development Program of China (863 Program) (Grant No. 2006AA10A102), National Natural Science Foundation of China (Grant No. 30600349) and Natural Science Foundation of Zhejiang Province (Grant No. Y306149)
  • 相关项目:水稻大叶角突变体的基因克隆及基因的功能研究
中文摘要:

狭窄的叶异种在在米饭变化 Zhonghua 11 上进行的 T-DNA 转变以后被获得。包括半矮子,几个反常词法特征推迟了 flowering 时间,狭窄、里面的滚动叶子,和更低的种子背景,被观察。网络光合作用的率(在下面浸透光) 在异种的标志叶子比野类型的显著地低。而且,在变异的旗帜叶的叶蒸发率和有气孔的传导力是比在充满舞台的谷物的野类型的那些低的。变异的显型没被 T-DNA 插入引起,这被发现。基因分析证明异种被单个后退的基因控制,指定了(t) 为 nal3。一张基因连接地图用 2 印射人口在 12 由 BAS 方法从 366 个 SSR 标记识别了的染色体上与 6 个多态的标记从在 nal3 (t) 和 indica 变化 Longtefu B 之间的一个十字导出的大 F 被构造。基因 nal3 (t) 在标记 RM7018 和 RM3331 之间被印射。nal3 (t) 地点的好印射在怀有在 Nipponbare 之间的 nal3 (t) 的区域附近基于顺序差异与 22 个最新发达的圣标记被进行并且 93 11,并且 nal3 (t) 最后被印射到在圣标记 NS10 和 RH12-8 之间的一个 136-kb 区域。

英文摘要:

A narrow leaf mutant was obtained after T-DNA transformation conducted on a rice variety Zhonghua 11. Several abnormal morphological characteristics, including semi-dwarf, delayed flowering time, narrow and inward rolling leaves, and lower seed-setting, were observed. The rate of net photosynthesis (under saturate light) of flag leaves in the mutant was significantly lower than that of the wild type. Moreover, the leaf transpiration rate and stomatal conductance in the mutant flag leaf were lower than those of the wild type at the grain filling stage. It was found that the mutant phenotype was not caused by the T-DNA insertion. Genetic analysis showed that the mutant was controlled by a single recessive gene, designated as nal3(f). A genetic linkage map was constructed using a large F2 mapping population derived from a cross between nal3(t) and an indica variety Longtefu B with 6 polymorphic markers on chromosome 12 identified from 366 SSR markers by the BAS method. Gene nal3(t) was mapped between the markers RM7018 and RM3331. Fine mapping of nal3(t) locus was conducted with 22 newly developed STS markers based on the sequence diversity around the region harboring nal3(t) between Nipponbare and 93-11, and nal3(t) was finally mapped to a 136-kb region between the STS markers NS10 and RH12-8.

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