以中亚热带常绿阔叶林主要优势树种丝栗栲(Castanopsis fargesii)、苦槠(Castanopsis sclerophylla)和青冈(Cyclobalanopsis glauca)幼苗为研究对象,通过MSR-2420红外辐射增温装置和人工氮输入,设置增温和不同施氮梯度(CK,0kg N/(hm2·a);N1,60kg N/(hm2·a);N2,120kg N/(hm2·a)),研究增温(W)和施氮(N1和N2)及其交互作用(WN1和WN2)对丝栗栲、苦槠和青冈叶片光合生理指标的影响。结果表明:(1)增温导致丝栗栲、苦槠和青冈叶片叶绿素a、叶绿素b、叶绿素总量、可溶性蛋白和可溶性糖含量总体呈现减小趋势,而叶绿素a/b和淀粉含量则表现为不同程度的增加;(2)施氮(N1和N2)引起丝栗栲、苦槠和青冈叶片叶绿素a、叶绿素b、叶绿素总量显著增加及其可溶性糖和淀粉含量显著降低。丝栗栲和苦槠叶片可溶性蛋白含量随氮输入量的增加而增加,而青冈则呈现降低趋势;(3)丝栗栲、苦槠和青冈幼苗叶片生理指标对增温和施氮交互作用(WN1和WN2)的响应规律与单独施氮时基本一致,但不同树种之间存在差异。以上研究结果说明,增温导致丝栗栲、苦槠和青冈叶片叶绿素含量减小,并抑制植物叶片光合代谢产物的积累,而施氮不但有利于其叶绿素含量的增加,同时促进其蛋白质的合成,增加其可溶性糖和淀粉的消耗。
Taking Castanopsis fargesii, Castanopsis sclerophylla and Cyclobalanopsis glauca seedings as the research objects, the dominant species of evergreen broad-leaved forest in the mid-subtropical zone, through MSR-2420 infrared radiation warming devices and nitrogen fertilizer application, air temperature warming and different nitrogen fertilizer addition level (CK,0 kg N/(hm2 · a); N1, 60 kg N/(hm2· a); N2, 120 kg N/(hm2· a)) was set up to investigate the effects of the warming (W), nitrogen fertilizer addition (N1 and N2)and their interaction (WN1 and WN2) on leaf photosynthetic physiological indexes. The results showed that: (1)Warming caused the decrease of Chl a, Chl b, total chlorophyll , soluble protein and soluble sugar contents while the Chl a/b and the content of starch increased. (2)Under the condition of nitrogen fertilizer addition, the increase of Chl a, Chl b, total chlorophyll contents and the decrease of soluble sugar and starch content were significant. The soluble protein content of Castanopsis fargesii and Castanopsis sclerophylla seedling leaves increased while Cyclobalanopsis glauca seedling leaves showed the reverse changing trend.(3) The interactive effects of warming and nitrogen addition on photosynthetic physiological indexes of three seedlings were consistent with nitrogen fertilizer addition. There were somewhat differences between WN1 and WN2 among different tree species. In a word, warming caused the decrease of Castanopsis fargesii, Castanopsis sclerophylla and Cyclobalanopsis glauca seedling leave chlorophyll content and restrained the accumulation of plant photosynthetic metabolite accumulation, and nitrogen addition promoted the protein synthesis and increased the consumption of soluble sugars and starch.