中文摘要：

目的观察镓铝砷（Ga Al A s）激光对脂肪源性干细胞（ADSCs）增殖、细胞因子分泌及成脂分化能力的影响,探讨临床治疗过程中提高ADSCs治疗效果的有效方法。方法取来自腹部脂肪抽吸术女性患者的脂肪组织,分离ADSCs,建立人脂肪源性干细胞（h ADSCs）培养体系;用650nm波长Ga Al As激光以不同能量（2、4、8J/cm2）对h ADSCs进行照射。采用MTT法检测h ADSCs的增殖能力,ELISA法检测h ADSCs的分泌功能,实时定量PCR法检测细胞表型的变化,油红O染色检测激光照射对h ADSCs成脂分化能力的影响。结果 MTT法检测结果显示：照射后第4、6天时2J/cm2照射组吸光度值与对照组无明显差异（P〉0.05）,4、8J/cm2照射组吸光度值与对照组比较差异有统计学意义（P〈0.05）,其中4J/cm2照射组吸光度差异最为明显。ELISA法检测结果显示：4J/cm2照射组上清液中血管内皮生长因子（VEGF）、血小板衍生生长因子（PDGF）、转化生长因子β（TGF-β）的蛋白含量分别为287.5±15.2pg/ml、36.0±0.7ng/ml、292.6±10.5pg/ml,明显高于对照组（分别为145.3±21.6pg/ml、26.7±0.6ng/ml、130.6±6.0pg/ml）,差异有统计学意义（P〈0.05）。荧光定量PCR检测结果显示：经4J/cm2 Ga Al As激光照射后h ADSCs中细胞表面标记物CD13、CD29、CD44、CD90 m RNA的相对表达量分别为对照组的1.19、22.05、4.38、7.22倍,两组间比较差异有统计学意义（P〈0.05）。油红O染色显示,经Ga Al As激光照射后h ADSCs的成脂分化能力明显高于对照组。结论低能量激光照射可增强ADSCs的增殖、分泌及成脂分化能力,可能有助于提高其临床治疗效果,是一种简便、安全、有效的方法。

英文摘要：

Objective To investigate the effects of low level gallium aluminum arsenide （GaA1As） laser irradiation on proliferation, cytokine secretion and adipogenic differentiation of cultured human adipose tissue-derived stem cells （hADSCs）, and to find a safe and effective method for improving clinical treatment effect of ADSC. Methods The cultured hADSCs from human adipose tissue were treated by using 650nm GaA1As laser irradiation at a single of 2, 4 and 8J/cm2 respectively. Cell proliferation was quantified by MTT assay, cytokine secretion was determined by ELISA, and adipogenic differentiation was examined by oil red staining~espectively. In addition, the expression profiles of putative hADSC surface markers were determined by real-time PCR. Results MTT assay showed that treatment with GaA1As laser irradiation at 4J/cm2 and 8J/cm2 （especially 4J/cm2） markedly promoted ADSCs proliferation from day 4 to day 6 after irradiationj when compared with the uuirradiated group （P〈0.05）. ELISA results showed that the concentrations of VEGF, PDGF and TGF- [3 in culture supernatant were 287.5 ~ 15.2pg/ml, 36.0 -＋ 0.7ng/ ml, and 292.6 ＋ 10.5pg/ml when hADSCs were exposed to 4J/cm2 GaAIAs laser, and they were significantly higher than those of unirradiated group （145.3 ＋ 21.6pg/ml, 26.7 -＋ 0.6ng/ml and 130.6 -＋ 6.0pg/ml, respectively, P〈0.05）. Quantitative PCR showed that the mRNA expression levels of cell surface marker CD13, CD29, CD44 and CD90 in hADSCs exposed to 4J/cm2 GaA1As laser were 1.19, 22.05, 4.38 and 7.22 fold of those in unirradiated group （P〈0.05）. And the oil red staining revealed that GaA1As laser irradiation could markedly accelerate the adipogenic differentiation of hADSCs （P〈0.05）. Conclusions Low energy laser irradiation is a simple, safe and effective method, which can enhance the proliferation, cytokine secretion and adipogenic differentiation of ADSCs, may help to improve the therapeutic effect of ADSCs by clinical transformation application.