中文摘要：

目的探讨重度子痫前期（sPE）患者合体滋养细胞表面微绒毛膜（STBM）脱落水平与胎盘组织Rho/ROCK分子表达的关系。方法收集sPE产妇（sPE组）和正常产妇（对照组）胎盘组织各20例,透射电镜下观察胎盘合体滋养细胞表面微绒毛结构变化,应用体视学方法分析细胞表面微绒毛数密度（Nv）、表面积密度（Sv）和体积密度（Vv）,通过Western blotting和免疫组化检测胎盘组织中Rho亚家族蛋白成员（RhoA、RhoB、RhoC）及Rho激酶（ROCKI、ROCKⅡ）的表达水平。应用Spearman等级相关分析法检验Rho、ROCK蛋白表达水平与STBM脱落水平的相关性。结果 sPE组患者胎盘合体滋养细胞表面微绒毛的Nv、Sv、Vv均显著小于对照组（P〈0.05）,胎盘组织中RhoA、RhoB、ROCKI、ROCKⅡ蛋白表达水平明显高于对照组（P〈0.05）。sPE组患者胎盘组织中RhoB、ROCKI、ROCKⅡ的表达水平与STBM的脱落水平均呈正相关（r=0.631,r=0.826,r=0.865,P〈0.05）。结论 RhoB及其与下游分子ROCKI、ROCKⅡ构成的信号通路可能在sPE时胎盘STBM脱落过程中发挥重要作用。

英文摘要：

Objective To study the relationship between syncytiotrophoblast microvillous membrane（STBM） shedding in severe preeclampsia（sPE） and placenta Rho/ROCK molecule expression.Methods In this study,placenta tissue of 20 sPE lying-in women（sPE group） and 20 normal lying-in women（control group） were collected.The syncytiotrophoblast microvillous structure of the placenta tissue was scrutinized with transmission electron microscope（TEM）.The microvillous numerical density（Nv）,surface density（Sv）,and volume density（Vv） of the cell surfaces were analyzed with stereology method.Western blot and immunohistochemistry were used to detect the expression of Rho subfamily proteins（RhoA,RhoB,RhoC） and Rho kinase（ROCKI,ROCKII） in the placenta tissues.The correlation of the expression of Rho,ROCK proteins,and the shedding of STBM was examined with Spearman rank correlation analysis.Results The Nv,Sv,and Vv of the syncytiotrophoblast microvillous membranes in sPE group were significantly less than those of the control group（P〈0.05）.Compared with the control group,the expression levels of RhoA,RhoB,ROCKI,and ROCKII proteins in sPE group were noticeably elevated（P〈0.05）.In sPE group,the expressions of RhoB,ROCKI,and ROCKII proteins were positively correlated with the shedding of STBM（r=0.631,r=0.826,r=0.865,P〈0.05）.Conclusion The signaling pathways constituted by RhoB and its downstream molecules ROCKI/ROCKII,may play an important regulatory role in the upregulation of STBM shedding in sPE.