中文摘要：

目的检测鞭毛蛋白诱导急性肺损伤（ALI）大鼠肺组织中Toll样受体5（TLR5）mRNA的表达变化，初步探讨TLR5在鞭毛蛋白致大鼠Au中的作用。方法从大肠杆菌ATCC25922中分离纯化鞭毛蛋白并鉴定。108只SD大鼠随机分为正常对照组（n=36）、致伤1组（n=36）和致伤2组（n=36）。经尾静脉注射鞭毛蛋白以建立大鼠ALI模型。每组分别在6个时相点用原位杂交法检测大鼠肺组织中TLR5 mRNA的表达，并观察动脉血气分析和肺病理改变。结果从大肠杆菌ATCC25922中成功分离纯化出鞭毛蛋白，其分子量约65kD。静脉注射鞭毛蛋白成功建立了大鼠急性肺损伤模型。从致伤后1h开始，鞭毛蛋白致肺损伤大鼠肺组织中TLR5 mRNA表达增加，并随时间推移和鞭毛蛋白剂量增加而表达增多。各致伤组大鼠动脉血氧分压降低，肺组织出现肺间质、肺泡水肿和炎性细胞浸润等病理改变。结论静脉注射鞭毛蛋白可导致大鼠ALI；TLR5作为细胞膜上鞭毛蛋白的受体参与了鞭毛蛋白致大鼠急性肺损伤的作用过程。

英文摘要：

Objective To determine the expression of Toll-like receptor 5（TLRS） mRNA in the lung tissues of rats with acute lung injury induced by flagellin, and to investigate its potential role in the pathogenesis of the disease. Methods Flagellin was isolated and puri- fied from Escherichia coli ATCC25922, subsequently identified by monoclonal antibody to flagellin. One hundred and eight SD rats were randomly divided into control group （n=36）, flagellin challenged 1 group （n=36） and flagellin challenged 2 group （n=36）. Rat model of ALI was reproduced by injecting flagellin. The expression of TLR5 mRNA in the lung tissues of rats was determined with in situ hybridization technique at six time points. Blood gas was monitored and pathological changes in the lung was observed at the same time. Result Flagellin was isolated and purified successfully and its molecular weight was approximately 65kD. Flagellin-induced acute lung injury model was successfully reproduced in rats. From lh after flagellin injection, TLR5 mRNA expression was found to be increased in the lung tissues of rats with flagellin induced acute lung injury, and the expression was on the increase with the elapse of time and increase of the dose of flagellin.The PaO2 levels in flagellin challenged 1 and 2 groups decreased. Interstitial edema, alveolar edema and inflammatory cells infiltration were also observed in flagellin challenged groups. Candusion Flagellin can induce ALI in rats; the development of flagellin induced ALI in rats is related with TLR5 expression in the lung tissues of rats.