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Differentially Expressed Genes in Heads and Tails of Angelica Sinensis diels: Focusing on Ferulic Acid Metabolism
  • ISSN号:1006-3250
  • 期刊名称:《中国中医基础医学杂志》
  • 分类:Q78[生物学—分子生物学] TS202.3[轻工技术与工程—食品科学;轻工技术与工程—食品科学与工程]
  • 作者机构:[1]Bijie Medical College, Bijie, Guizhou Province (551700), China, [2]Department of Fundamental Medicine, Chengdu University of Traditional Chinese Medicine, Chengdu (610075), China
  • 相关基金:Supported by the National Natural Science Foundation of China(No.81273899)
中文摘要:

探索在当归 sinensis diels 的头和尾巴之间的差异的药品的活动的科学涵义的目的(作为) ,广泛地在中国药利用的重要植物,由定序的 transcriptome 的途径。方法十件样品这在 Min 县是随机镇定的,中国的 Gansu 省。Transcriptome 定序是在一个商业 ILLumina HiSeq-2000 平台被完成。每个头的 transcriptome 和尾巴是在一个基因薄片被修理,并且在 Illumina HiSeq-2000 的过程下面检测了。差别表示了在头和尾巴之间的 unigenes 是被上海生物工学公司(SBC ) 在网上选择分析系统基于基因本体论,基因和染色体和相关生物信息的数据库的京都百科全书。结果完全, 63,585 unigenes 从作为由高产量的定序被获得站台。在哪个之中当差别表示了在头和尾巴之间的 unigenes, 3359 unigenes 被识别作为由 SBC 分析系统扫描。哪个 15 差别表示了 unigenes 参予 phenylpropanoid 生合成(PB ) 的新陈代谢的规定小径和 ferulic 酸代谢物,响应头的著名药品的行动和尾巴作为。在头和尾巴的 ferulic 酸的结论不同内容这与差别有关表示基因,特别地在 PB 小径包含了。

英文摘要:

Objective: To explore the scientific connotation of the discrepant pharmaceutical activities between the head and tail of Angelica sinensis diels(AS), an important herb extensively utilized in Chinese medicine, by the approach of transcriptome sequencing. Methods: Ten samples of AS were randomly collected in Min County, Gansu Province of China. Transcriptome sequencing of AS was accomplished in a commercial ILLumina Hi Seq-2000 platform. The transcriptome of each head and tail of AS were fixed in a gene chip, and detected under the procedure of Illumina Hi Seq-2000. Differentially expressed unigenes between the heads and tails of AS were selected by Shanghai Biotechnology Corporation(SBC) online analysis system, based on Gene Ontology, Kyoto Encyclopedia of Genes and Genomes and relevant bioinformatic database. Results: Totally 63,585 unigenes were obtained from AS by high-throughput sequencing platform. Among which 3359 unigenes were identified as differentially expressed unigenes between the heads and tails of AS by SBC analysis system scanning. Of which 15 differentially expressed unigenes participate in the metabolic regulation of phenylpropanoid biosynthesis(PB) pathway and ferulic acid metabolites, in response to the distinguished pharmaceutical actions of the heads and tails of AS. Conclusion: Different content of ferulic acid in the heads and tails of AS is related to the differentially expressed genes, particularly involved in the PB pathway.

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期刊信息
  • 《中国中医基础医学杂志》
  • 北大核心期刊(2011版)
  • 主管单位:国家中医药管理局
  • 主办单位:中国中医研科学院中医基础理论研究所
  • 主编:孟庆云
  • 地址:北京东直门内南小街16号
  • 邮编:100700
  • 邮箱:
  • 电话:010-64089056
  • 国际标准刊号:ISSN:1006-3250
  • 国内统一刊号:ISSN:11-3554/R
  • 邮发代号:80-330
  • 获奖情况:
  • 国家科技部中国科技论文统计源期刊,中国学术期刊综合评价数据库来源期刊
  • 国内外数据库收录:
  • 美国化学文摘(网络版),中国中国科技核心期刊,中国北大核心期刊(2004版),中国北大核心期刊(2008版),中国北大核心期刊(2011版),中国北大核心期刊(2014版)
  • 被引量:36850