探索在当归 sinensis diels 的头和尾巴之间的差异的药品的活动的科学涵义的目的(作为) ,广泛地在中国药利用的重要植物,由定序的 transcriptome 的途径。方法十件样品这在 Min 县是随机镇定的,中国的 Gansu 省。Transcriptome 定序是在一个商业 ILLumina HiSeq-2000 平台被完成。每个头的 transcriptome 和尾巴是在一个基因薄片被修理,并且在 Illumina HiSeq-2000 的过程下面检测了。差别表示了在头和尾巴之间的 unigenes 是被上海生物工学公司(SBC ) 在网上选择分析系统基于基因本体论,基因和染色体和相关生物信息的数据库的京都百科全书。结果完全, 63,585 unigenes 从作为由高产量的定序被获得站台。在哪个之中当差别表示了在头和尾巴之间的 unigenes, 3359 unigenes 被识别作为由 SBC 分析系统扫描。哪个 15 差别表示了 unigenes 参予 phenylpropanoid 生合成(PB ) 的新陈代谢的规定小径和 ferulic 酸代谢物,响应头的著名药品的行动和尾巴作为。在头和尾巴的 ferulic 酸的结论不同内容这与差别有关表示基因,特别地在 PB 小径包含了。
Objective: To explore the scientific connotation of the discrepant pharmaceutical activities between the head and tail of Angelica sinensis diels(AS), an important herb extensively utilized in Chinese medicine, by the approach of transcriptome sequencing. Methods: Ten samples of AS were randomly collected in Min County, Gansu Province of China. Transcriptome sequencing of AS was accomplished in a commercial ILLumina Hi Seq-2000 platform. The transcriptome of each head and tail of AS were fixed in a gene chip, and detected under the procedure of Illumina Hi Seq-2000. Differentially expressed unigenes between the heads and tails of AS were selected by Shanghai Biotechnology Corporation(SBC) online analysis system, based on Gene Ontology, Kyoto Encyclopedia of Genes and Genomes and relevant bioinformatic database. Results: Totally 63,585 unigenes were obtained from AS by high-throughput sequencing platform. Among which 3359 unigenes were identified as differentially expressed unigenes between the heads and tails of AS by SBC analysis system scanning. Of which 15 differentially expressed unigenes participate in the metabolic regulation of phenylpropanoid biosynthesis(PB) pathway and ferulic acid metabolites, in response to the distinguished pharmaceutical actions of the heads and tails of AS. Conclusion: Different content of ferulic acid in the heads and tails of AS is related to the differentially expressed genes, particularly involved in the PB pathway.