中文摘要：

目的 观察表皮生长因子受体（EGFR）在非小细胞肺癌（NSCLC）患者手术前后血清水平变化的情况.方法 采用酶联免疫吸附试验（ELISA）检测78例NSCLC、127例良性肺疾病患者和205例健康体检者血清中EGFR的含量,以ROC曲线分析确定血清EGFR的Cut-off值,探讨NSCLC术前EGFR含量与临床病理指标、生存情况的关系,并根据手术前后血清EGFR含量的变化情况分析其在预后判断中的应用价值.结果 NSCLC组术前血清中EGFR含量高于良性对照组组和健康对照组（P＜0.05）.ROC曲线分析EGFR的Cut-off值为6.75μg/L.logistic回归分析显示,有吸烟史、较高的TNM分期和出现淋巴结转移是NSCLS患者术前血清EGFR升高的危险因素.生存分析表明术前EGFR高表达组和术后EGFR＞ 6.75μg/L的患者生存时间显著缩短（P＜0.05）.Cox比例风险回归模型分析表明术前较高的血清EGFR水平是影响患者生存时间的独立危险因素.结论 对于接受肺癌根治术治疗的患者,检测术后早期血清EGFR含量变化对于预后判断有较好的应用价值.

英文摘要：

Objective To observe the changes of serum epidermal growth factor receptor （EGFR） level in pa- tients with non - small cell lung cancer （NSCLC） before and after operation. Methods Serum EGFR contents of 78 pa- tients with NSCLC （group A）, 127 patients with benign pulmonary disease （group B） and 205 healthy people taking medical examination （ group C） were detected with enzyme linked immunosorbent assay （ ELISA）, and the Cut - off val- ues of serum EGFR were analyzed with ROC curve. The relationships between preoperative EGFR levels with clinical pathological indexes and survival rate were investigated, and the value of the serum EGFR content in prognosis was ana- lyzed according to the changes before and after the operation. Results Preoperative serum EGFR content in group A was significantly increased compared with those in group B and C （ P 〈 0.05 ）. The Cut - off value of EGFR analyzed with the ROC curve was 6.75 μg/L. Logistic regression analysis showed that the history of smoking, higher TNM stage and lymph node metastasis were the risk factors of high serum EGFR level of NSCLS patients. Survival analysis showed that survival time was significantly shortened in patients with preoperative high EGFR expression and patients with postoperative EGFR 〉 6.75 μg/L （P 〈 0. 05）. Cox proportional hazards regression models showed that the preoperative high serum EGFR level was an independent factor of influencing the survival time. Conclusion It is valuable to detect postoperative early changes of serum EGFR content for lung cancer patients undergoing radical resection.