中文摘要：

以红莲型水稻不育系粤泰A（YTA）线粒体DNA为模板,在具有细胞质遗传的线粒体特异性片段HL-sp1侧翼各设计3条特异性巢式引物（LSP1﹑LSP2﹑LSP3﹑RSP1﹑RSP2、和RSP3）,利用特异性引物和随机引物AD1﹑AD2和AD3,通过热交错PCR（TAIL-PCR）扩增HL-sp1的侧翼序列。通过扩增和序列分析得到了HL-sp1 5′侧翼1 456 bp和3′侧翼2 570 bp的序列。改进后的TAIL-PCR方法为线粒体目的片段的侧翼序列分析提供了一种简单而高效的方法。

英文摘要：

Mitochondrial genomic DNA of Yuetai A-Hong-lian cytoplasmic male sterility （HL-CMS） lines were taken as model,and three nested special primers sets were designed in the end region of HL-spl,which were named LSP1,LSP2,LSP3, RSP1 ,RSP2 and RSP3. Improved TAIL-PCR technique was used to amplify the flanking sequence of HL-spl utilizing those three nested sequence specific primers with a shorter arbitrary degenerated primer （AD1,AD2,AD3）, respectively. The amplifying results and the sequence analysis indicated that a 1 456 bp flanking sequence of 5-end and a 2 570 bp flanking sequence of 3-end of HL-spl were obtained. TAIL-PCR provided a simple and efficient method for the cloning of the HL-spl flanking sequences.