本文通过固相萃取结合分子络合物一分散液液微萃取,与高效液相色谱联用,建立了一种测定椰子汁中酸性植物激素的新方法。选择了几种典型酸性植物激素吲哚乙酸、水杨酸、脱落酸和吲哚丁酸作为分析物,考察了该方法的萃取性能。在固相萃取与分子络合物一分散液液微萃取联用模式中,椰子汁中分析物首先吸附在C-18萃取材料上,待解吸完成后,解吸液又可用作分散液液微萃取的分散剂,大大简化了萃取步骤。该方法的富集倍数可达319~478倍,线性关系良好,具有良好的精密度和准确度,有望用于植物激素的检测。
A new method combining solid-phase extraction(SPE) and molecular complex-based dispersive liquid-liquid microextraction(MC-DLLME) with high performance liquid chromatography(HPLC) has been developed for the determination of several acidic phytohormones in natural coconut juice. Indole-3- acetic acid (IAA), salicylic acid (SA), abscisic acid (ABA) and indolyl-3-butyricacid (IBA) were selected to investigate the extraction performance and the method sensitivity. In solid phase extraction-dispersive liquid-liquid microextraction(SPE-DLLME) procedure, analytes were firstly adsorbed onto C18 sorbent from a large volume of coconut juice samples, the eluate containing the desired compounds was used as the dispersive solvent in MC-DLLME procedure, and extraction step was largely simplified. The new method provided a high enrichment factor (319-478) for several acidic phytohormones. Good linearities were obtained for the analytes, and the developed method has also good precision and recovery, which provides application potential in the analysis of phytohormones.