中文摘要：

冬虫夏草是一种被人们普遍接受的药用真菌。由于其生长缓慢,培养条件苛刻,故难以大规模人工培养应用。本文以实验室分离得到的虫草菌株Ophiocordyceps sinens is CCTCC M2016041为研究对象,通过正交试验设计对影响虫草菌株在大米培养基上生长的光照、温度和菌液接种量这3个因素进行9 d的观察,每3 d记录菌体的增重量,以确定大米培养基培育虫草的最佳生长条件,并测量其甘露醇含量。结果表明,3个影响因子光照、温度和菌液接种量的重要性在生长初期（前3 d）依次为光照〉温度〉菌液接种量;在生长中期（3～6 d）为温度〉光照〉菌液接种量;在生长后期（6～9 d）为光照〉温度〉菌液接种量。培养虫草菌的最适条件为光照24 h、培养温度25℃、1：1（mL：g）的菌米接种体积重量比。而米基培养的M2016041菌株菌丝中虫草酸含量为（8.42±0.45）mg/g。本试验能够为人工模拟自然条件下虫草的大规模培养提供一种有效的技术方法。

英文摘要：

Ophiocordyceps sinensis is one kind of widely accepted medical fungus.However,it is hard to cultivate artificially for the large scale application because of its slow growth rate and fastidious cultivation conditions.In this study,taking Ophiocordyceps sinersis CCTCC M2016041 reserved in laboratory as test material to study its biological characteristics and the optimal cultivation conditions on rice medium.By means of orthognnal design of experiments on the effects of Ophioeordyceps strain on the rice medium,three factors including light,temperature and inoculum size were observed for 9 clays ,weight recording every 3 days ,in order to tetermine the optimum growth condition.Meanwhile the content of mannitol in the hyphae was determined.It suggested that in the initial growth stage （before 3 days） ,the range values of three factors were 0.098,0.057 and 0.022,and the ranks of importance was following ：light 〉 temperature 〉 inoculum size.In the middle growth stage （3-6 clays ）, the ranks of importance was following ： temperature〉 light 〉 inoculum size.In the late growth stage （6-9 days）,the ranks of importance was following ：light 〉 temperature 〉 inoculum size.The optimum conditions for culturing eordyceps sinensis were light time 24 h, culture temperature 25 ℃, and volume weight ratio of bacteria and rice as 1：1. Results were showed that the content of mannose in the hyphae was （8.42±0.45） mg/g.Conclusively, this study could provide an effective technical met- hod which could be artificially simulated under natural conditions for large-scale cultivation of Ophiocordyceps.