为研究西府海棠的黄酮类成分及含量,采用硝酸铝-亚硝酸钠-氢氧化钠法显色,采用分光光度法测定了西府海棠叶总黄酮的含量,优选出西府海棠总黄酮的最佳提取条件.利用乙酸乙酯和石油醚分别对样品进行萃取纯化,以层析比较其黄酮种类.结果表明:西府海棠叶总黄酮最佳提取工艺为固液比1:20,乙醇浓度70%,提取时间4h,提取温度80℃.微乳膜薄层层析显示,西府海棠叶中含有多种黄酮类,其中有芦丁、槲皮素、葛根素.
To investigate the kinds of flavonoids in leaves of Malus micromalus Makino,flavonoids was colored by the method of aluminum nitrate and sodium nitrite colorimetric,and the content of flavonoids was determined by spectrophotometry,the optimized extraction system of total flavonoids in M.micromalus was thus determined by the means of orthogonal experimental design.And then,flavonoids purified by ethyl acetate or petroleum ether were analyzed by microemulsion TLC.The results showed that the most proper fators to extract total flavonoids from leaves of M.micromalus were:70%ethanol extracting for 4h,at the extraction temperature of 80℃,in the extracting system with solid:liquid=1:30.As TLC shown,the extraction of ethyl acetate contains many kinds of flavonoids,3of them are rutinum,quercetin and puerarin.