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中文摘要：
建立了液液萃取耦合气相色谱/质谱联用（GC/MS/MS）分析斑马鱼卵中10种多溴联苯醚（PBDEs）,12种甲氧基化多溴联苯醚（MeO-PBDEs）和9种羟基化多溴联苯醚（HO-PBDEs）不同极性组分的同步分析方法。优化了前处理步骤和色谱质谱条件,采用液液萃取法提取目标化合物,不必分离即可实现目标化合物的衍生化,使样品前处理时间大大缩短,实现了不同极性组分化合物的同步分析。采用干法制备酸性硅胶柱纯化,用15 mL正己烷和15 mL二氯甲烷正己烷（1：1,V/V）混合溶剂洗脱效果良好。31种化合物在4.0～500μg/L浓度范周内线性良好,相关系数均大于0.99,回收率在71.6%～133.0%之间;相对标准偏差低于21.5%;检出限在0.09～17.0 ng/g之间。本方法应用到斑马鱼亲子代间转移研究中,对实际样品的检测良好,并发现BDE-47,6-HO-BDE-47,6-MeO-BDE-47,2＇-HO BDE-28和2＇-MeC-BDE 28可通过母代斑马鱼暴露后,不同程度地转移到子代中。
英文摘要：
An analytical method was developed for the simultaneous analysis of 10 kinds of PBDEs,12 MeO-PBDEs and 9 kinds of HO-PBDEs in zebrafish eggs by liquid-liquid extraction coupled with GC/ MS/MS.The conditions of extraction,chromatography and mass spectrometry were optimized.The target compounds were extracted through liquid-liquid extraction and derivatization of HO-PBDEs can be carried out without separation from extraction solution.Thus the pre-treatment time could be shortened and all target compounds could be analyzed simultaneously.Purification was conducted by dry packed acidic silica gel column.All compounds can be eluted with 15mL n-hexane and 15mL of dichloromethane and hexane（1：1,V/V） mixed solvent.Under optimized conditions,31 compounds had good linearity in concentration range from 4.0 to 500μg/L,with correlation coefficient（R~2 ） better than 0.9933,recoveries in the range of 71.6%—133.0%with relative standard deviations less than 21.5%,and the method and instrument detection limits varied from 0.09 to 17.0 ng/g.The presented method was applied for maternal transfer study of BDE-47,6-HOBDE-47, 6-Me（）-BDE-47,2＇-HO-BDE-28 and 2＇-MeO-BDE-28 and turned out to be efficient for real sample analysis.After maternal exposure,the five compounds showed different maternal transfer potency in zebrafish,giving new consideration for the exposure assessment of such compounds.
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