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汉族人早年白发差异表达基因的初步筛选及功能探索
  • ISSN号:1673-6273
  • 期刊名称:《现代生物医学进展》
  • 分类:R758.71[医药卫生—皮肤病学与性病学;医药卫生—临床医学] Q78[生物学—分子生物学]
  • 作者机构:[1]第四军医大学西京医院皮肤科,陕西西安710032
  • 相关基金:国家自然科学基金项目(81000701); 2010年中华医学会-欧莱雅中国人健康皮肤/毛发研究项目资助
中文摘要:

目的:通过筛选早年白发患者和正常黑发者毛发中差异表达的基因,为研究毛发黑素合成及转运的生理病理机制提供线索。方法:留取门诊确诊的早年白发患者毛发,应用退火控制引物(ACP)初步筛选差异表达的候选基因,实时定量PCR法进一步确认候选基因的表达水平;设计合成筛选出的Slc7a11和小眼相关转录因子(Mitf)的siRNA后,转染入正常人黑素细胞系PIG1,在H2O2诱导下,通过Annexin-V FITC检测其凋亡。结果:ACP筛选及RT-PCR验证结果提示,Slc7a11和Mitf基因表达水平在早年白发患者和正常人间有较大差异(P〈0.01);降低这两种基因的表达后,PIG1的凋亡率较正常组升高。结论:Slc7a11和Mitf基因不仅在正常毛发色素合成中发挥着作用,同时也参与着氧化应激诱导下黑素细胞凋亡的过程,可能在一些色素相关性疾病的发生发展中起着关键的作用。

英文摘要:

Objective: To investigate the various genes between premature greying of hair patients and healthy subjects by screening,which implied a way for understanding the pathogenesis of melanin genesis and transport.Methods: Hair of premature greying patients were collected,and annealing control primers(ACP) were preliminarily used to screen for candidate genes,then the expression level of these genes were confirmed through Real-time PCR.siRNA were used to transfect into PIG1 cell lines,then the apoptosis rates were analyzed by AnnexinV-FITC,under H2O2-induced oxidative stress.Results: There was significance in both Slc7a11 and Mitf genes expression,between patients and healthy ones(P0.01).When these two genes were down regulated,the apoptosis rate of PIG1 increased upon oxidative stress.Conclusion: Slc7a11 and Mitf are major genetic regulators of melanin pigment in hair and provide more evidence for the further investigation of pigmentation disorder,including the process of oxidative stress-induced melanocytes apoptosis.

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期刊信息
  • 《现代生物医学进展》
  • 中国科技核心期刊
  • 主管单位:黑龙江省卫生厅
  • 主办单位:黑龙江省红十字医院 黑黑龙江省红十字医院 黑龙江省森林工总医院
  • 主编:申宝忠
  • 地址:哈尔滨市南岗区花园街184号403
  • 邮编:150001
  • 邮箱:biomed_54@126.com
  • 电话:0451-82583800 53658268
  • 国际标准刊号:ISSN:1673-6273
  • 国内统一刊号:ISSN:23-1544/R
  • 邮发代号:14-12
  • 获奖情况:
  • 国内外数据库收录:
  • 被引量:33230